Phenotype and Molecular Characterization of Antibiotic Resistance of Salmonella spp. from Cattle in Abidjan District, Côte d'Ivoire

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Coulibaly Kalpy Julien
Yao Kouamé René
Dagnogo Komissiri
Tiékoura Konan Bertin
Koffi Kouassi Eugène
Ajayi Abraham
Djaman Allico Joseph
Dosso Mireille
Yapi Houphouët Félix


Aims: The overall objective of this study was to study antibiotic-resistant strains of Salmonella in livestock sales sites in the Abidjan district.

Place and Duration of Study: Researchers team from Institute Pasteur of Ivory Coast and their students collected samples of cattle feces in five townships of the district of Abidjan from April to September 2016.

Methodology: Fresh cow dung has been collected from sales outlets and livestock pens in five municipalities in Abidjan. The prevalence of Salmonella carrying has been studied by classical microbiological techniques. These included strain isolation by culture on the Hektoen medium, biochemical identification using Leminor's reduced rack and strain confirmation by MALDI-TOF MS. Phenotypic determination of antibiotic resistance and detection their genes were carried out respectively by the method of discs diffusion on the Muller-Hinton agar and using PCR simplex.

Results: In this study, we collected 420 samples.

The results showed that the overall prevalence of Salmonella isolated from cattle feceswas 20% (84/420). Twenty-six (26) strains were resistant to at least one antibiotic. The blaCTX-M, blaSHV, blaTEM and TetA resistance genes were detected with the respective frequencies of 7.7%, 57.7% and 7.7%.

Conclusion: The isolation of antibiotic-resistant Salmonella strains in these healthy cattle poses a significant threat to public health. However, a good use of antibiotics in farms could help limit the phenomenon of resistance of Salmonella to antibiotics.

Salmonella spp, antibiotics, resistance, cattle, Abidjan, Côte d'Ivoire.

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How to Cite
Julien, C. K., René, Y. K., Komissiri, D., Konan Bertin, T., Eugène, K. K., Abraham, A., Joseph, D. A., Mireille, D., & Félix, Y. H. (2019). Phenotype and Molecular Characterization of Antibiotic Resistance of Salmonella spp. from Cattle in Abidjan District, Côte d’Ivoire. Journal of Advances in Microbiology, 18(4), 1-10.
Original Research Article


Moyaert H, De Jong A, Simjee S. Antimicrobial resistance monitoring projects for zoonotic and indicator bacteria of animal origin: Common aspects and differences between EASSA and EFSA. 2014;171:279–283.

Alexander KA, Warnick LD, Wiedmann M. Antimicrobial resistant Salmonella in dairy cattle in the United States, Vet. Res. Commun. 2009;33(3):191–209.

Kemal J. A review on the public health importance of Bovine Salmonellosis. J. Vet. Sci. Technol. 2014;05(2):1-10 .

Addis Z, Kebede N, Worku Z, Gezahegn H, Yirsaw A, Kassa T. Prevalence and antimicrobial resistance of Salmonella isolated from lactating cows and in contact humans in dairy farms of Addis Ababa: A cross sectional study. BMC Infect. Dis. 2011;11(22):1-7.

Yang B, Qu D, Zhang X, Shen J, Cui S, Shi Y, et al. International Journal of Food Microbiology Prevalence and characterization of Salmonella serovars in retail meats of marketplace in Shaanxi, China, Int. J. Food Microbiol. 2010;141(1-2):63–72.

Pribul BR, Festivo ML, de Souza MMS, dos Rodrigues DP. Characterization of quinolone resistance in Salmonella spp. isolates from food products and human samples in Brazil. Braz. J. Microbiol. 2015;47(2016):196–201.

CDC, Antibiotic resistance threats in the United States; 2013.


EFSA and ECDC. The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2017. EFSA J. 2019;17(2):5598.

Coulibaly KJ, Koffi S, Dou S, Bamba A, N’douba AK, Dosso M. The antibiotic resisting profile of Salmonella spp. Isolated from the Sewage of the Campus of the University of Cocody, Abidjan, Côte d’ Ivoire. 2014;4(5):608–620.

Coulibaly KJ, Stephane K, Serge D, Aboubacar B, N'douba KA, Mireille D. Prevalence of Antibiotics Resistant Salmonella in the Abidjan North Wastewater in 2012 and Potential Health Risks to the Population. J. Adv. Microbiol. 2016;1(1):1-9.

Bonny A, Karou A, Sanogo M, Atobla Ahonzo-Niamke KS. Prevalence of Salmonella and their antibiotic susceptibility patterns in the District of Abidjan, Côte d’Ivoire. Int. J. Biol. Chem. Sci. 2019;8(2):450-458.

Koffi AR, Ouassa T, Dadie A, Karou T, Koffi MDJE. Sérotypes et profils d’ antibio-résistance de Salmonella suspectées d’ origine alimentaire et isolées chez des patients diarrhéiques à Abidjan, Côte d’ Ivoire. 2012;56:336-342.

Koffi AR, Adjehi D, Tago K, Marcelin DK. Original research article serotypes and antibiotic resistance of Salmonella spp. isolated from poultry carcass and raw gizzard sold in markets and catering in Abidjan, Côte d’ Ivoire. 2014;3(6):764–772.

Lee VT, Smith RS, Tümmler B, Lory S. Activities of Pseudomonas aeruginosa effectors secreted by the type III secretion system in vitro and during infection. Infect. Immun. 2005;73(3):1695–1705.

Serghini MA, Ritzenthaler C, Pinck L. A rapid and efficient miniprep for isolation of plasmid DNA. Press Nucleic Acids Res. 1989;17(9):3604.

Olesen I, Hasman H, Aarestrup FM. Prevalence of B-lactamases among ampicillin-resistant Escherichia coli and Salmonella isolated from food animals in Denmark. Microb. Drug Resist. 2004;10(4):334–340.

Moodley A, Guardabassi L, Transmission of IncN Plasmids Carrying bla CTX-M-1 between Commensal Escherichia coli in Pigs and Farm Workers †,” Antimicrob. Agents Chemother. 2009;53(4):1709–1711.

Birkett CI, Ludlam HA, Woodford N, Brown DFJ, Brown NM, Roberts MTM, et al. Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum b-lactamases. J. Med. Microbiol. 2007:56:52–55.

Yagi T, Kurokawa H, Shibata N, Shibayama K, Arakawa Y. A preliminary survey of extended-spectrum v-lactamases (ESBLs) in clinical isolates of Klebsiella pneumoniae and Escherichia coli in Japan. FEMS Microbiol. Lett. 2000;184:53–56.

Waters SH, Rogowsky P, Grinsted J, Altenbuchner J, Schmitt R. Nucleic acids research the tetracydine resistance determinants of RP1 and Tnl721: nucleotide sequence analysis. Nucleic Acids Res. 1983;11(17):6089–6105.

Alemayehu D, Molla B, Muckle A. Prevalence and antimicrobial resistance pattern of Salmonella isolates from apparently healthy slaughtered cattle in Ethiopia. Trop. Anim. Health Prod. 2003;35(4):309–319.

Eguale T, Engidawork E, Gebreyes WA, Asrat D, Alemayehu H, Medhin G, et al. Fecal prevalence, serotype distribution and antimicrobial resistance of Salmonellae in dairy cattle in central Ethiopia. BMC Microbiol. 2016;16(1):20.

Nyabundi D, Onkoba N, Kimathi R, Nyachieo A, Juma G, Kinyanjui P, et al. Molecular characterization and antibiotic resistance profiles of Salmonella isolated from fecal matter of domestic animals and animal products in Nairobi. Trop. Dis. Travel Med. Vaccines. 2017;3:2.

Ishihara K, Takahashi T, Morioka A, Kojima A, Kijima M, Asai T, et al. National surveillance of Salmonella enterica in food-producing animals in Japan. Acta Vet. Scand. 2009;51:35.

Milnes AS, Sayers AR, Stewart I, Clifton-Hadley FA, Davies RH, Newell DG, et al. Factors related to the carriage of Verocytotoxigenic E. coli, Salmonella, thermophilic Campylobacter and Yersinia enterocolitica in cattle, sheep and pigs at slaughter. Epidemiol. Infect. 2009;137(8): 1135–1148.

Akoachere JFTK, Tanih NF, Ndip LM, Ndip RN. Phenotypic characterization of Salmonella typhimurium isolates from food-animals and abattoir drains in Buea, Cameroon. J. Heal. Popul. Nutr. 2009;27(5):612–618.

Kagambèga A, Lienemann T, Aulu L, Traoré AS, Barro N, Siitonen A, et al. Prevalence and characterization of Salmonella enterica from the feces of cattle, poultry, swine and hedgehogs in Burkina Faso and their comparison to human Salmonella isolates. BMC Microbiol. 2013;13(1):253.

Turpin PE, Maycroft KA, Rowlands CL, Wellington EMH. Viable but non-culturable Salmonellas in soil. J. Appl. Bacteriol. 1993;74(4):421–427.

Madec J, Haenni M, Jouy E, Granier S, Weill F, Le Hello S. Les entérobcatéries résistantes aux céphalosporines de dernières générations générations : de l’animal à l’Homme,” Bull. Epidémiologique, Santé Anim. Aliment. 2012;53(1):37–39.

Dutta S, Scientist G, Sudhanthirakodi S, Jain P, Chattopadhyay UK. Non-typhoidal Salmonella isolates from livestock and food samples, Kolkata, India Conclusion: High prevalence of NTS in poultry and meat samples in Kolkata poses a potential risk of foodborne disease in humans. J. Microbiol. Infect. Dis.2016;66(33):113–120.

Yu T, Jiang X, Zhou Q, Wu J, Wu Z. Antimicrobial resistance, class 1 integrons, and horizontal transfer in Salmonella isolated from retail food in Henan, China. J. Infect. Dev. Ctries. 2014;8(6):705–711.

Mihaiu L, Lapusan A, Tanasuica R, Sobolu R, Mihaiu R, Oniga O, et al. First study of Salmonella in meat in Romania. J. Infect. Dev. Ctries. 2014;8(1):050-058.

White DG, Zhao S, Sudler R, Ayers S, Friedman S, Chen SG, et al. The isolation of antibiotic-resistant Salmonella from retail ground meats. New Englang J. Med. 2001;345(16):1147–1154.

Gorman R, Adley CC. Nalidixic acid-resistant strains of Salmonella showing decreased susceptibility to fluoroquinolones in the mid-west region of the Republic of Ireland. J. Antimicrob. Chemother. 2003;51:1047–1049.

Sanjukta R, Dutta JB, Sen A, Shakuntala I, Ghatak S, Puro AK, et al. Characterization of multidrug-resistant Escherichia coli and Salmonella isolated from food producing animals in Northeastern India. Int. J. Infect. Dis. 2016;45:114–115.

Smith AB, Renter DG, Cernicchiaro N, Shi X, Nagaraja TG. Prevalence and quinolone susceptibilities of Salmonella isolated from the feces of preharvest cattle within feedlots that used a fluoroquinolone to treat bovine respiratory disease. Foodborne Pathog. Dis. 2016;13(6):303–308.

Nyabundi D, Onkoba N, Kimathi R, Nyachieo A, Juma G, Kinyanjui P, et al. Molecular characterization and antibiotic resistance profiles of Salmonella isolated from fecal matter of domestic animals and animal products in Nairobi. Trop. Dis. Travel Med. Vaccines. 2017;3(1):2.

Murugkar HV, Rahman H, Kumar A, Bhattacharyya D. Isolation, phage typing and antibiogram of Salmonella from man and animals in northeastern India. Indian J. Med. Res. 2005;122(3):237–42.

Mihaiu L, Lapusan A, Tanasuica R, Sobolu R, Mihaiu R, Oniga O, et al. First study of Salmonella in meat in Romania. J. Infect. Dev. Ctries. 2014;8(1):050-058.

Edrington TS, Hume ME, Looper ML, Schultz CL, Fitzgerald AC, Callaway TR E, et al. Variation in the faecal shedding of Salmonella and E. coli O157:H7 in lactating dairy cattle and examination of Salmonella genotypes using pulsed-field gel electrophoresis. Lett. Appl. Microbiol. 2004;38(5):366–372.

Umeh SI, Paulinus EC. Antimicrobial resistance profile of Salmonella isolates from livestock. Open J. Med. Microbiol. 2014;4(4):242–248.

Dargatz DA, Fedorka-Cray PJ, Ladely SR, Kopral CA, Ferris KE, Headrick ML. Prevalence and antimicrobial susceptibility of Salmonella spp. isolates from US cattle in feedlots in 1999 and 2000. J. Appl. Microbiol. 2003;95(4):753–761.

Igbinosa IH. Prevalence and detection of antibiotic-resistant determinant in Salmonella isolated from food-producing animals. Trop. Anim. Health Prod. 2014;47 (1):37–43.

Ouattara N, Guessennd N, Gbonon V, Toe E, Dadié T, Tiécoura B. Consommation des antibiotiques dans la filière aviaire à Abidjan: Cas de quelques fermes semi-industrielles. Eur. J. Sci. Res. 2013;94:80–85.

de Jong A, Thomas V, Simjee S, Godinho K, Schiessl B, Klein U, et al. A pan-European survey of antimicrobial susceptibility towards human-use antimicrobial drugs among zoonotic and commensal enteric bacteria isolated from healthy food-producing animals. J. Antimicrob. Chemother. 2009;63(4);733–744.

Huang X, Yu L, Chen X, Zhi C, Yao X, Liu Y, et al. High prevalence of colistin resistance and mcr-1 gene in Escherichia coli isolated from food animals in China. Front. Microbiol. 2017;8:1-5.

Kempf I, Fleury MA, Drider D, Bruneau M, Sanders P, Chauvin C, et al. What do we know about resistance to colistin in Enterobacteriaceae in avian and pig production in Europe? Int. J. Antimicrob. Agents. 2013;42(5):379–383.

Kempf I, Jouy E, Chauvin C. Colistin use and colistin resistance in bacteria from animals. Int. J. Antimicrob. Agents. 2016;48(6):598–606.

Lertworapreecha M, Noomee S, Sutthimusik S, Utarapichat B, Tontikapong K. Multidrug resistant and extended spectrum B-lactamase producing Salmonella enterica isolated from food animals in phatthalung, Thailand. Southeast Asian J. Trop. Med. Public Health. 2016;47(6):1257–1269.

Koo HJ, Woo GJ. Distribution and transferability of tetracycline resistance determinants in Escherichia coli isolated from meat and meat products. Int. J. Food Microbiol. 2011;145(2-3):407–413.