Spatial Distribution of Bacterial Isolates from Different Abattoirs in Port Harcourt, Nigeria

Main Article Content

Azuonwu Testimonies Chikanka
David N. Ogbonna

Abstract

The constituents of wastes generated from abattoir activities create conducive environment for microbial proliferation, most of which are pathogenic. Infections caused by these microorganisms could result to zoonoses. This study was to determine the distribution of bacterial isolates and their biomass from different abattoirs in Port Harcourt. Samples like waste blood, table swab, service water, faecal matter, soil and wastewater from abattoirs in Iwofe, Rumuodomaya and Trans-Amadi were collected from October 2017 to November, 2018 and analysed using standard microbiological procedures. Results obtained revealed that the total heterotrophic bacterial count of blood samples ranged from 8.33x101 to 3.33x102 cfu/ml for Trans-Amadi and Iwofe abattoirs, table swabs ranged from 6.74x104 to 4.88x106 cfu/ml, water samples ranged from 1.56x104 to 2.07x104 cfu/ml; faecal matter had THB counts ranging from 9.97x107 to 1.06x108 cfu/g; and soil samples ranged from 1.11x1010 to 1.17x1010 g,  while wastewater counts ranged from 1.03x108 to 1.08x108 cfu/ml. The predominant Bacterial isolates were of the genera Micrococcus, Staphylococcus, Serratia, Pseudomonas, Proteus, Klebsiella, Escherichia and Chromobacterium, Serratia sp. only was isolated from Iwofe and Rumuodomaya abattoirs within April to October while Chromobacterium sp. was isolated in Trans-Amadi and Rumuodomaya abattoirs within the months of May to October. Among the isolates, Escherichia coli and Klebsiella species occurred more compared to others in all the three locations. A higher percentage of microorganisms were recorded in the month of May compared to other months. It is presumed that abattoir wastes harbour many microorganisms of public health importance. The occurrence of these microbes, most of which are enteric pathogens, poses a public health challenge as infections by them could result in illnesses such as gastroenteritis, septicaemia and pneumonia especially in the absence of good hygiene around abattoirs. Proper sanitation in abattoirs as well as management of abattoir wastes is important in reducing the spread of these microorganisms.

Keywords:
Abattoir, microorganisms, infections, environmental sanitation.

Article Details

How to Cite
Testimonies Chikanka, A., & N. Ogbonna, D. (2020). Spatial Distribution of Bacterial Isolates from Different Abattoirs in Port Harcourt, Nigeria. Journal of Advances in Microbiology, 19(4), 1-8. https://doi.org/10.9734/jamb/2019/v19i430201
Section
Original Research Article

References

Fereidoun H, Nourddin MS, Rreza NA, Mohsen A, Ahmad R, Pouria H. The effect of long-term exposure to particulate pollution on the lung function of Teheranian and Zanjanian students. Pakistan Journal of Physiology. 2007;2: 1-5.

RMMA (Red Meat Abattoir Assoiction). Waste Management in Red Meat Abattoir, Pretoria, South Africa. 2010;40.

Adelegan JA. Environmental policy and Slaughter house waste in Nigeria. IN: Reed, B. (ed). Sustainable environmental sanitation and water services: Proceedings of the 28th WEDC International Conference, Kolkata (Calcutta), India. 2002;4.

Sangodoyin AY, Agbawhe OM. Environmental Study on surface and ground water pollutant from abattoir effluents. Bioresource Technology. 1992; 41(3):193-200.

Adedipe NO. The challenge of Urban solid waste management in Africa, IN: Baijnath. H and Singh (Eds) Rebirth of science in Africa (Hatfield, South Africa: Umdaus Press). 2002;175-192.

Akindele ST, Abimbola WA, Oluwabiyi BA, Jokotagba OA, Agbolade OM, Sam-Wobo SO. Microbiological assessment of waste-water and soil in Ijebu-Igbo Abattoir, Southwest, Nigeria. International Journal of Advancements in Research & Technology. 2015;4:10

Cadmus SI, Olugasa BO, Ogunipe GA. The prevalence and zoonotic importance of bovine tuberculosis in Ibadan. Proceedings of 37th Annual Congress of the Nigerian Veterinary Medical Association. 1999;883-886.

Ogbonna DN, Azuonwu TC. Plasmid profile and antibiotic resistance pattern of bacteria from abattoirs in Port Harcourt City, Nigeria. International Journal of Pathogen Research. 2019;2(2):1-11.

Ire FS, Amos MO, Ossia-Ndidi LN. Microbiological and physiochemical assessment of abattoir effluents and receiving water bodies in Port Harcourt. Journal of Pharmaceutical, Clinical and Biological Sciences. 2017;5 (1):34-39.

Igbinosa EO, Obuekwe IS. Evaluation of antibiotic resistant gene in abattoir environment. Journal of Applied Sciences and Environmental Management. 2014; 18(2):5-9.

Azuonwu TC, Ogbonna DN. Resistance genes of microbes associated with abattoir wastes. Journal of Advances in Medical and Pharmaceutical Sciences. 2019;21(2): 1-11.

Cheesbrough M. District laboratory practice in tropical countries. Part 2. Cambridge University Press. 2006; 143-157.

Ezeronye OU, Ubalua AO. Studies in the effect of abattoir and industrial effluents on the heavy metals and microbial quality of Aba River in Nigeria. African Journal of Biotechnology. 2005;4(3):266-272.

Adesemoye AO, Opere BO, Makinde SCO. Microbial content of abattoir wastewater and its contaminated soil in Lagos, Nigeria, African Journal of Biotechnology. 2006; 5(20):1963-1968.

Teo KC, Teoh SM. Preliminary biological screening of microbes isolated from cow dung in Kampar. African Journal of Biotechnology. 2011;10(9):1640-1645.

Adekanmbi AO, Falodun OI. Heavy metals and antibiotics susceptibility profiles of Staphylococcus aureus isolated from several points receiving daily input from the Bodija Abattoir in Ibadan, Oyo State, Nigeria. Advances in Microbiology. 2015; 5:871-880.

Onuoha CS Distribution and antibiogram of bacterial species in effluents from abattoirs in Nigeria. Journal of Environmental and Occupational Science. 2018;7(1):1-8.

Nandita D, Badaru OA, Adekeye BT. Microbiological assessment of agege Abattoir situated In Lagos State, Nigeria. IOSR Journal of Environmental Science, Toxicology and Food Technology. 2015; 9(9):86-93.

Faria D, Bharath L. Marine and estuarine methylotrophs: Their abundance activity and identity. Current Science. 2006;90(7): 984-989.

Nafarnda WD, Ajaylu JC, Kawe MS, Omeiza GK, Sani NA, Tenuche OZ, Dantong DD, Tags SZ. Bacteriological quality of abattoir effluents discharged into water bodies in Abuja, Nigeria. Veterinary Science. 2012;515689.

Ogbonna DN. Distribution of microorganisms in water, soils and sediment from abattoir wastes in Southern Nigeria. Int. J. Curr. Microbiol. App. Sci. 2014;3(9):1183-1200.

Adeyemo OK, Ayodeji IO, Aiki-raji CO. The water quality and sanitary conditions in a major abattoir (Bodija) in Ibadan, Nigeria, Afr. J. Biomedical Res. 2002;5:51-55.

Armand-Lefevre L, Ruimy R, Andremont A. Clonal comparison of Staphylococcus aureus isolates from healthy pig farmers, human controls, and pigs. Emerging Infectious Diseases. 2005;11(5):711-714.

Schlech WF, Lavigne PM, Bortolussi RA. Epidemic listeriosis-evidence for transmission by food. The New England Journal of Medicine. 2005;308:203-206.

Adesoji AT, Abubakar F, Ipinlaye JS. Occurrence of antibiotic resistant bacteria in faeces from Abattoir waste, processing water and products from Dutsin-Ma, Katsina State, Nigeria. Journal of Bacteriology and Mycology. 2016;3(1): 1022.

Amisu KO, Coker AO, Isokphehi RD. Arcobacter butzlieri strains from poultry abattoir effluent in Nigeria. East Afr. Med. J. 2003;80:218-221.

Byamukama D, Farnleither AH, Kansiime F, Manafi M, Burtsher M, Mach RL. Contrasting occurrence of Chromobacterium violaceum in tropical drinking water springs of Uganda. Journal of Water Health. 2005;3:229e38.

Yang C, Li Y. Chromobacterium violaceum infection: A clinical review of an important but neglected infection. Journal of Chinese Medical Association. 2011;74:435-461.

Khanna A, Khanna M, Aggarwal A. Serratia marcescens- A rare opportunistic nosocomial pathogen and measures to limit its spread in hospitalized patients. Journal of Clinical and Diagnostic Research. 2013;7(2):243-246.

Rajpit A, Shah U, Chauhan B, Shah P. Serratia: An emerging pathogen in hospital environment. Gujarat Medical Journal. 2009;64(2):70-7.