Microbiological and Physicochemical Evaluation of Oil-polluted Soil from Major Auto Mechanic Shops in Port Harcourt Metropolis, Rivers State, Nigeria

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Janet Olufunmilayo Williams
Prekebi Peter Akemi

Abstract

The capability of microorganisms to utilize spent oil in polluted soil from some major auto mechanic workshops in Port Harcourt metropolis as the sole source of carbon and energy was studied. Soil samples were collected from three (3) Auto mechanic workshops (Mile 3 Mechanic Village (N 4º48' 34.07'', E 6º59' 10.17''), Ikoku Mechanic Workshop (N 4º47' 54.28'', E 6º59' 36.42'') and Elekahia Zone H Mechanic Workshop (N4º49' 11.62'', E 7º1' 16.58'') in Port Harcourt, Rivers State, Nigeria from depths of 0–30 cm, 30–60 cm and 60–90 cm at the same spot from each station. Soil samples were analysed microbiologically and physicochemically using standard methods. The control sample was obtained behind the Biology Building of Rivers State University, Port Harcourt. The results of the total heterotrophic bacterial and fungal counts showed that the microbial load was high at a depth of 0 to 30 cm for all the samples analysed including the control. With 60-90 cm depth, low microbial counts were obtained. The total heterotrophic bacterial counts from the three mechanic workshops ranged from 6.8 X 108 to 2.3 X 109cfu/g while the total heterotrophic fungal counts ranged from 1.3 X 106 to 8.0X 106 cfu/g. The spent oil-utilizing microbial populations ranged from1.0 X 105 to 3.0 X 106cfu/g and1.0 X 105 to 8.0 X 105 cfu/g for the bacterial and fungal counts respectively. Five spent oil utilizing bacterial isolates of the genera, Pseudomonas, Klebsiella, Bacillus, Micrococcus and Proteus and five fungal isolates of the genera, Penicillium, Candida, Rhizopus, Fusarium and Aspergillus were obtained from spent oil-polluted soil in this study. The pollution index of the different physicochemical parameters obtained from the different sample stations was as follows: Electrical Conductivity (EC): Mile 3 had the highest with the mean of 12.50 ±0.0-13.20±0.0µs/cm, while Ikoku had the lowest with mean of 10.99 ± 0.0-11.50 ± 0.0 µs/cm. Ikoku had the highest pH level with mean of 7.0 ± 0.0- 8.5 ± 0.0 while Mile 3 had the lowest pH level with mean of 6.5 ± 0.0-7.59 ± 0.0. Ikoku had the highest Total Organic Carbon (TOC) with mean of 17.55 ± 0.0-20.60 ± 0.0 mg/kg while Elekahia had the lowest with the mean of 16.99 ± 0.0-18.0 ± 0.0 mg/kg. Ikoku had the highest Total Hydrocarbon Content (THC) with mean of 38,862 ± 0.0-40,500 ± 0.0 mg/kg while Mile 3 had the lowest with the mean of 20,550 ± 0.0-30,000 ± 0.0 mg/kg. From the Pollution Index Analysis, Ikoku Auto Mechanic Workshop is more contaminated with Spent oil than Mile 3 and Elekahia.

Keywords:
Spent oil, polluted soil, microbial isolates, automechanic workshops, physicochemical parameters.

Article Details

How to Cite
Williams, J. O., & Akemi, P. P. (2020). Microbiological and Physicochemical Evaluation of Oil-polluted Soil from Major Auto Mechanic Shops in Port Harcourt Metropolis, Rivers State, Nigeria. Journal of Advances in Microbiology, 20(3), 1-10. https://doi.org/10.9734/jamb/2020/v20i330222
Section
Original Research Article

References

Lale OO, Ezekwe IC, Lale NES. Effect of spent lubricating oil pollution on some chemical parameters and the growth of cowpeas (Vigna unguiculata Walpers) Resources and Environment. 2014;43:173-179.

Helmenstine AM. Chemical composition of petroleum; 2013.
Available:http://chemical.about.com/od/geochemistry/a/chemicalcomposition of petroleum.htm

Akinpelumi BE, Olatunji OA. Effects of sawdust-soil amendment on the soil growth and yield of Solanum esculentum in waste engine oil polluted soil. Sciences in Cold and Arid Regions. 2015;7(2):128-136.

Adams GO, Tawari–Fufeyin P, Ehinomen I. Laboratory scale bioremediation of soils from automechanic workshops using cowdung. Journal of Applied & Environmental Microbiology. 2014;2(4): 129 –134.

Husaini A, Roslan HA, Hi KSY, Ang CH. Biodegradation of aliphatic hydrocarbon by indigenous fungi isolated from used motor oil contaminated sites. World Journal of Microbiology and Biotechnology. 2008;24 (12):2789-2797.

Kvenvolden KA, Cooper CK. Natural seepage of crude oil into the marine environment. Geo-Marine Letters. 2003;23 (3-4):140-146.

Franco I, Contin M, Bragato G, De Nobili M. Microbiological resilience of soils contaminated with crude oil. Geoderma. 2004;121:17-30.

Erdogan E, Karaca A. Bioremediation of crude oil polluted soils. Asian Journal of Biotechnology. 2011;3:206-213.

Odjegba VJ, Sadiq AO. Effect of spent engine oil on the growth parameters, chlorophyll and protein levels of Amaranthus hybridus. The Environmentalist. 2002;22:23-28.

Olugboji OA, Ogunwole OA. Use of spent engine oil. Assumption University Journal of Technology. 2008;2(1):67-71.

Akoachere JFTK, Akanji TN, Yongabi FN, Nkwelang G, Ndip RN. Lubricating oil degrading bacteria in soils from filling stations and Automechanic workshops in Buea, Cameroon: Occurrence and characteristics of isolates. African Journal of Biotechnology. 2008;7:1700-1708.

Adelowo OO, Alagbe SO, Ayandele AA. Time-dependent stability of used engine oil degradation by cultured of Pseudomonas fragi and Achromobacter aerogenes. African Journal of Biotechnology. 2006;5: 2476-2479.

Buchanan RE, Gibbsons NE. Bergey’s manual of determinative bacteriology. 8th edition, Baltimore, Williams & Wilkins Co; 1974.

Williams JO, Wilcox IM. Utilization of phenol by microorganisms isolated from petroleum polluted soil in Port Harcourt, Rivers State. Asian Journal of Science and Technology. 2018;9(10):8913-8920.

Odokuma LO, Williams JO. A Mathematical model incorporating the influence of Biodegradation on the fate of a simulated oil spill in a Brackish Aquatic System. British Journal of Environment and Climate Change. 2012;2(1):73-98.

Obire OE, Anyanwu C, Okigbo RN. Saprophytic and crude oil-degrading fungi from cow dung and poultry droppings as bioremediating agents. International Journal of Agricultural Technology. 2008;4 (2):81-89.

Okpokwasili GC, Amanchukwu SC. Petroleum hydrocarbon degradation by Candida species. Environmental International Journal. 1988;14:243- 245.

American Public Health Association. Standard methods for the examination of water and waste water, 16th ed. American Public Health Association, New York; 1998.

Umanu G, Omoikhudu AR. Oil degradation assessment of bacterial isolates from motor oil contaminated soil in Ota Nigeria. International Journal of Advanced Biological Research. 2013;3(4):506 -513.

Khan JA, Rizvi SHA. Isolation and characterization of microorganisms from oil contaminated sites. Advances in Applied Science Research. 2011;2(3):455-460.

Abioye OP, Agamuthu P, Abdul Aziz AR. Biodegradation of used motor oil in soilusing organic waste. Biotechnology Research International. 2012;8(10):1-9.

Ogunbayo AO, Bello RA, Nwagbara U. Bioremediation of engine oil contaminated site. Journal of Emerging Trends in Engineering and Applied Sciences. 2012; 3(5):483-489.

April TM, Foght JM, Currah RS. Hydrocarbon-degrading filamentous fungi isolated from flare pit soils in Northern and Western Canada. Canadian Journal of Microbiology. 2000;46(1):38-49.

George-Okafor U, Tasie F, Muotoe-Okafor F. Hydrocarbon degradation potentials of indigenous fungal isolates from petroleum contaminated soils. Journal of Physical and Applied Sciences. 2009;3:1-6.