Physicochemical, Bacteriological and Parasitological Examination of Selected Fish Pond Water Samples in Awka and Its Environment Anambra State, Nigeria

Main Article Content

Umeh Odera Richard
E. I. Chukwura
Ibo Eziafakaego Mercy

Abstract

A fish pond with recommended water quality will produce healthy fishes. Fish ponds with poor water quality will cause fish mortality and outbreak of diseases to fish consumers. Physicochemical analysis was done using standard analytical methods, the total bacterial count was determined by dilution and membrane filtration techniques. Parasitological analysis was done using the centrifugation method. A total of fifteen well waters were sampled during wet season. Results showed that the temperature ranged from 27°C to 29°C, pH, 6.21 to 8.15; dissolved oxygen, 4.28 mg/l to 5.78 mg/l, electrical conductivity, 166.36 µs/cm to 394.00 µs/cm; total dissolved solids, 41 mg/l to 121 mg/l; total suspended solids, 1.00 mg/l to 19.40 mg/l; total solids, 42.00 mg/l to 140.4 mg/l; turbidity values, 7.01 NTU to 10.36 NTU; nitrate, 3.10 mg/l to 28.00 mg/l; total alkalinity, 36 mg/l to 91 mg/l; phosphate, 1.26 mg/l to 13.11 mg/l; sulphate, 0.39 mg/l to 4.37 mg/l; total chloride, 7.08 mg/l to 14.19 mg/l; carbonates, 1.33 mg/l to 2.35 mg/l; bicarbonates, 34.59 mg/l to 89.38 mg/l; total hardness, 25.31 mg/l to 53.04 mg/l; calcium hardness, 23.94 mg/l to 51.96 mg/l; magnesium hardness, 1.08 mg/l to 4.20 mg/l; total acidity, 2 mg/l to 22 mg/l; potassium, 0.04 mg/l to 2.23 mg/l; cadmium, 0.00 mg/l to 0.04 mg/l; lead, 0.01 mg/l - 0.16 mg/l; chromium, 0.00 mg/l - 0.03 mg/l; mercury was not detected, copper, 0.00 mg/l - 0.04 mg/l; arsenic, 0.00 mg/l - 0.02 mg/l; zinc, 0.00 mg/l to 0.02 mg/l; iron, 0.01 mg/l - 1.19 mg/l. The total bacterial counts ranged from 3.60-4.12 log cfu/ml; total coliforms, 14-46 cfu/100ml, Vibrio cholerae, 0-11 cfu/100ml; Vibrio parahaemolyticus, 0-15 cfu/100ml; faecal coliform, 1-9 cfu/100 ml; Acinetobacter calcoaceticus, 0-8 cfu/100 ml; Bacillus subtilis, 0-9 cfu/ml; Staphylococcus aureus, 0-5 cfu/ml; Pseudomonas aeruginosa, 0-12 cfu/100 ml; Pseudomonas fluorescens, 0-12 cfu/100 ml and Clostridium perfringens were not detected in any of the samples. Twelve bacterial species namely Klebsiella pneumoniae, Acinetobacter calcoaceticus, Escherichia coli, Staphylococcus aureus, Vibrio cholerae, Pseudomonas fluorescens, Pseudomonas aeruginosa, Proteus mirabilis, Vibrio parahaemolyticus, Bacillus subtilis, Shigella flexineri and Salmonella typhi were isolated and identified using standard analytical and molecular procedures. Parasites identified were Ichthyobodo species, Diplostomum species, Myxobolus species, Chilodonella species, Bothriocephalus species, Ambiphrya species and Leech species. Salmonella typhi had the highest frequency of isolation (20.63%) while Acinetobacter calcoaceticus and Staphylococcus aureus had the lowest frequency of isolation (2.83%). Ichthyobodo species had the highest frequency of isolation (21.43%) while Leech species had the lowest frequency of isolation (5.71%). Some of the physicochemical, bacteriological and parasitological parameters had values above World Health Organization admissible limits and therefore proper sanitary practices and water treatments must be employed to prevent epidemic among fish consumers.

Keywords:
Sanitary risk assessment, physicochemical, bacteriological, parasitological and fish pond waters.

Article Details

How to Cite
Richard, U. O., Chukwura, E. I., & Mercy, I. E. (2020). Physicochemical, Bacteriological and Parasitological Examination of Selected Fish Pond Water Samples in Awka and Its Environment Anambra State, Nigeria. Journal of Advances in Microbiology, 20(3), 27-48. https://doi.org/10.9734/jamb/2020/v20i330226
Section
Original Research Article

References

FAO (Food and Agriculture Organization of the United Nations). The state of world fisheries and aquaculture contributing to food security and nutrition for all, Rome. 2016;200.

Emikpe BO, Adebisi T, Adedeji OB. Bacteria load on the skin and stomach of Clarias gariepinus and Oreochromis niloticus from Ibadan, South West Nigeria. Public health implications. Journal of Microbiology and Biotechnology Research. 2011;1(1):52-59.

Ida CJ. Heavy metals in suchindramkulam (a lentic water body) of Kanyakumari District, Tamil Nadu, India. Journal of Tropical and Experimental Biology. 2012;8(3-4):141-145.

Adebayo-Tayo BC, Odu NN, Igiwiloh NJPN, Okonko IO. Microbiological and physicochemical level of fresh catfish (Arius hendelotic) from different markets in Akwa Ibom State, Nigeria. New York Science Journal. 2012b;5(4):46-52.

Noga EJ. Fish diseases, diagnosis and treatment. 2nd Edn. Iowa State University, Press, Ames, U.S.A. 2000;17-20.

Ikpi G, Offem B. Bacterial infection of mudfish Clarias gariepinus (Siluriformes: Clariidae) fingerlings in tropical nursery ponds. Journal of Revised Biology and Tropical. 2011;59(2):751-759.

Boon JH, Huisman EA. Viral, bacterial and fungal diseases of Siluroidei, cultured for human consumption. Aquatic Living Resource. 1996;9:153-164.

Dutta C, Saha D, Panigrahi AK, Sengupta C. The occurrence of Escherichia coli in fish samples isolated from different ponds of Nadia District, West Bengal, India. Internet Journal of Food Safety. 2010;12: 181-186.

APHA. American Public Health Association. Standard methods for the examination of water and wastewater, 21st Edn. Washington. 2005;1-21.

Cheesbrough M. District laboratory practice in tropical countries. Part 2. 2nd Edn. Cambridge University Press. South Africa. 2010;434.

Fafioye OO. Preliminary studies on water and bacterial population in high yield Kajola fish ponds. Journal of Agriculture Extension and Rural Development. 2011;3: 68 –71.

Torimiro N, Bebe PT, Ogundipe FE, Esan DM, Aduwo AI. The bacteriology and physicochemical analysis of freshwater fish ponds. International Research Journal of Microbiology (IRJM). 2014;5(3):28-32.

Ehiagbonare JE, Ogunrinde YO. Physico-chemical analysis of fish pond water in Okada and its environments, Nigeria. African Journal of Biotechnology. 2010; 9(36):5922-5928.

Babatunde BN, Woke GN. Analysis of the physicochemical burden of Oyo State Fish Pond, Ibadan, Southwest Nigeria. Journal of Applied Science and Environmental Management. 2015;19(2):259-264.

Ogeneogaga OI, Solomon RJ. Physico-chemical and bactriological investigation of selected fish pond in Kuje Area Council, Nigeria. Researcher. 2017;9(4):31-45.

World Health Organization (WHO). Guidelines for drinking water quality. Recommendation WHO, Geneva. 2006; 569-571.

Sipaúba-Tavares LH, Guariglia CST, Braga FMS. Effects of rainfall on water quality in six sequentially disposed fishponds with continuous water flow. Brazilian Journal of Biology. 2007;67(4): 643-649.

Eze VC, Ogbaran IO. Microbiological and physicochemical characteristics of fish pond water in Uphill, Delta State, Nigeria. International Journal of Current Research. 2010;8:82-87.

Mahananda MR, Mohanty BP, Behera NR. Physicochemical analysis of surface and groundwater of Bargarh District, Orissa, India. International Journal of Research and Review in Applied Sciences. 2010; 2(3):284-295.

Peter Kabiro Laibu. Determination of bacterial composition, heavy metal contamination and physico-chemical parameters of fish pond water in Abothuguchi Central, Meru County, Kenya. (Published Master’s Degree Thesis) School of Pure and Applied Sciences of Kenyatta University; 2015.

Agbaire PO, Akporido SO, Emoyan OO. Determination of some physicochemical parameters of water from artificial concrete fish ponds in Abraka and its environments, Delta State, Nigeria. International Journal of Plant, Animal and Environmental Sciences. 2015;5(3):70-76.

Bhatnagar A, Devi P. Water quality guidance for the management of pond fish culture. International Journal of Environ-mental Sciences. 2013;3(6):1980-1993.

Nduka JK, Orisakwe OE, Ezenweke LO. Some physicochemical parameter of potable water supply in Warri, Niger Delta Area of Nigeria. Scientific Research and Essay. 2008;3(11):547-551.

Dhavran A, Karu S. Pig dung as pond manure: Effect on water quality pond productively and growth of Carps in Poly Culture System. The International Centre for Living Aquatic Resources Management (ICLARM) Quarterly, Manila. 2002;25(1):1-14.

Thilza IB, Muhammad T. The effects of management practices on the physical and chemical water qualities and its possible implications on fish health in Maiduguri Metropolis. Researcher. 2010;2(11):15-23.

Singh MR, Gupta A, Beateswari KH. Physicochemical properties of water samples for Manipur river system. Indian Journal of Applied Science and Environmental Management (JASEM). 2010;14(4):85-89.

Santhosh B, Singh NP. Guidelines for water quality management for fish culture in Tripura. ICAR Research Complex for NEH Region, Tripura Centre Publication No. 29; 2007.

Duruibe JO, Ogwuegbu MOC, Egwurugwu JN. Heavy metal pollution and human biotoxic effects. International Journal of Physical Sciences. 2007;2(5):112-118.

Danyal I, Blake F, Andrea W, Rusyniak DE. Heavy metal poisoning: Clinical presentations and pathophysiology. Clinical Medicine. 2006;26:67-97.

Wegu MO, Akanimor JO. Assessment of heavy metal profile of the New Calabar River and its impact on juvenile Clarias gariepinus. Chemical Biodiversity. 2006;3: 79-87.

Chukwuka CO. Physicochemical and heavy metal assessment of water from selected boreholes In Kaura-Namoda Local Government Area, Zamfara State, Nigeria. Master of Science (M.Sc.) Thesis Ahmadu Bello University, Zaria, Nigeria; 2015.

Asolker SR, Sridhar T, Joshi SG. Pollution effect in drinking water. International Journal of Environmental Management. 2002;25:66-70.

Kori-siakpere O, Ubogu EO. Sublethal haematological effects of zinc on the freshwater fish, Heteroclarias sp. (Osteichthyes: Clariidae). African Journal of Biotechnology. 2008;7(12):2068–2073.

Javed M, Usmani N. Assessment of heavy metal (Cu, Ni, Fe, Co, Mn, Cr and Zn) pollution in effluent dominated rivulet water and their effect on glycogen metabolism and histology of Mastacembelus armatus, Springer Open Journal. 2013;2:1-13.

Silapajarn K, Boyd CE, Silapajarn O. Physical and chemical characteristics of pond water and bottom soil in channel catfish ponds in west-central Alabama. Bulletin. 2004;655:1-46.

Ozuturk M, Ozozen G, Minareci O, Minareci E. Determination of heavy metals in fish, water and sediments of Avsar Dam Lake in Turkey. Iran Journal of Environ-mental Health. Science and Engineering. 2009;6(2):73-80.

Okpokwasili GC, Ogbulie JN. Microbial and proximate composition of fish feed used in Nigeria aquaculture. Journal of Nature and Science Count of Sri Lanka; 1999.

Daboor SM. Microbiolological profile of El-Qanater. El-Khairia fish farm. Global Veterinarian. 2008;2:51-55.

Ampofo JA, Clerk GC. Diversity of bacteria contaminants in tissues of fish cultured in organic waste-fertilized ponds. Health implications. The Open Fish Science Journal. 2010;3:142-146.

Njoku OE, Agwa OK, Ibiene AA. An investigation of the microbiological and physicochemical profile of some fish pond water within the Niger Delta region of Nigeria. European Journal of Food Science and Technology. 2015;3(4):20-31.

Adedayo OA, Anthony IO. Bacteriological analysis of selected fish pond water samples in Akungba Akoko, Ondo State. Journal of Food, Agriculture and Environ-ment. 2014;12(2):1260-1265.

Madanire-Moyo G, Barson M. Diversity of metazoan parasites of the African catfish Clarias gariepinus (Gurchell, 1822) as indicators of pollution in a subtropical African river system. Journal of Helminthology. 2010;84:216-227.

Roberts LS, Janovy Jr. J. Foundations of parasitology (Seventh Edn). New York: McGraw-Hill. 2005;53.

Sigei Wilfred Kiprono. Fish parasites and fisheries productivity in relation to extreme flooding of Lake Baringo, Kenya. Published Master’s Thesis. Kenyatta University; 2017.

Bichi AH, Dawaki SS. A survey of ectoparasites on the gills, skin and fins of Oreochromis niloticus at Bagauda fish farm, Kano, Nigeria. Bayero Journal of Pure and Applied Sciences. 2010;3(1):83-86.