Genetic Characterization of Gram-negative Bacilli Encoding the blaNDM Resistance Gene
Lionel Eliada Benoit BAMBARA
*
Department Biochemistry - Microbiology Laboratory of Molecular Biology and Genetics (LAMBIOGEN), Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Amana METUOR DABIRE
Daniel OUEZZIN COULIBALY University, BP 176 Dedougou, Burkina Faso.
Rabietou NIKIEMA
Department Biochemistry - Microbiology Laboratory of Molecular Biology and Genetics (LAMBIOGEN), Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Olawoumi Fabrice KOUTA
Department Biochemistry - Microbiology Laboratory of Molecular Biology and Genetics (LAMBIOGEN), Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Olivia Rhaina BADINI
Department Biochemistry - Microbiology Laboratory of Molecular Biology and Genetics (LAMBIOGEN), Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Carbapenem-resistant Enterobacteriaceae strains, a major group of pathogenic bacteria, have become an increasingly common causative agent of nosocomial infections. The global spread of NDM-producing Enterobacteriaceae and the emergence of mobile genetic elements among a wide range of species have made these organisms a worldwide public health threat in recent years.
Objective: To characterize the genetic support of Gram-negative bacilli coding for blaNDM
Methodology: A sample of 278 bacterial strains was collected from Saint-Camille Hospital, Our Lady of Peace Polyclinic, and Pietro Annigoni Biomolecular Research Center in Ouagadougou. The genetic material of the bacterial strains was extracted by the boiling method. Plasmids were extracted by an alkaline lysis method. These DNAs are analyzed by conventional PCR to detect the NDM gene. Chi-square test is performed between chromosomes and plasmids and the significance will be approved at p < 0.05.
Results: In total, 33.4% or 93 strains coded for blaNDM in the total DNA (Chromosomes/Plasmids). Among these 93 strains, 16 strains that code for blaNDM or 17.2% whose genetic support is the plasmid. The majority species was E. coli, of which 37.8% coded for blaNDM. Chi-square test and Cramer's V test reveal values of 6.43 and 0.15 respectively with a P-value of 0.01.
Conclusion: Our study reveals that there is a relationship between chromosomes and plasmids in the transmission of the blaNDM resistance gene.
Keywords: BGN, chromosomes, plasmids, blaNDM, antibiotics