Genetic and Molecular Characterization of Biofilm Formation in Multidrug-Resistant Strains Pseudomonas aeruginosa and Staphylococcus aureus
Comoé Koffi Donatien BENIE *
Department of Biosciences, Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, University of Félix Houphouët Boigny, Abidjan, Côte d’Ivoire and Department of Bacteriology and Virology, Institut Pasteur of Côte d'Ivoire (IPCI), Abidjan, Côte d’Ivoire.
Yao Paul ATTIEN
Department of Biochemistry-Microbiology, Laboratory of Agrovalorisation, University of Jean Lorougnon Guede, Daloa, Côte d’Ivoire.
Haziz Orou SINA
Department of Science and Technology, Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou, Benin.
Koua ATOBLA
Department of Biosciences, Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, University of Félix Houphouët Boigny, Abidjan, Côte d’Ivoire.
Aya Carole BONNY
Department of Biosciences, Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, University of Félix Houphouët Boigny, Abidjan, Côte d’Ivoire.
Etienne DAKO
University of Moncton, School of Food Science, Nutrition, and Family Studies Faculty of Health Sciences and Community Services, NB Canada E1A 3E9, Canada.
Nathalie GUESSENND
Department of Bacteriology and Virology, Institut Pasteur of Côte d'Ivoire (IPCI), Abidjan, Côte d’Ivoire.
Lamine BABA-MOUSSA
Department of Science and Technology, Laboratory of Biology and Molecular Typing in Microbiology, University of Abomey-Calavi, Cotonou, Benin.
Adjehi DADIE
Department of Food Science and Technology, Laboratory of Biotechnology and Food Microbiology (LMBM), University of Nangui-Abrogoua, Abidjan, Côte d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
The virulence and antimicrobial resistance (AMR) of biofilm-forming bacteria pose a growing threat to public health. The aim of this study was to identify molecular determinants of resistance and biofilm formation in P. aeruginosa and S. aureus. A total of 120 strains consisting of P. aeruginosa (60) and S. aureus (60) were isolated from animal products. These strains underwent phenotypic, biochemical, and molecular identification. The molecular determinants of resistance (blaSHV, blaIMP, and blaTEM) and biofilm formation (PpyR, PslA, and PelA) were detected using the PCR method. In descending order of importance, the prevalence of resistance genes detected in P. aeruginosa was 63.3% (blaSHV), 53.3% (blaIMP), and 35.0% (blaTEM). S. aureus strains producing coagulase (83.33%) and DNase (75%) harbored blaIMP (48.3%) and blaSHV (36.7%) resistance genes with varying prevalences. The PpyR gene (93.3%) was predominantly detected in P. aeruginosa, followed by the PslA (80.0%) and PelA (71.7%) genes. Only the PslA (48.3%) and PelA (38.3%) genes were detected in S. aureus, with prevalences below 50.0%. Detecting and controlling the determinants of resistance and biofilm formation can guide improved diagnosis and contribute to the fight against bacterial biofilm infections.
Keywords: Biofilms, resistance, gene, P. aeruginosa, S. aureus