Activity of Honey and Propolis on Bacteria Isolated from Diabetic Foot
Hend Shubar
Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, Tripoli, Libya.
Manal Abdul Gawad
Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, Tripoli, Libya.
Basma Doro *
Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, Tripoli, Libya.
Najib Sufya
Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, Tripoli, Libya.
Attiya Alatery
Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, Tripoli, Libya.
Salah B. Mohamed
Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, Tripoli, Libya.
Najat Al Magrha
Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, Tripoli, Libya.
*Author to whom correspondence should be addressed.
Abstract
Aims: Diabetic foot ulcer is a significant complication of diabetes mellitus and often proceed lower extremely amputation. Propolis is a naturally occurring anti-inflammatory bee derived protectant resin. Previously, topically applied propolis has been reported to reduce inflammation and improves cutaneous ulcer healing in diabetic rodents. This study aimed to determine the Libyan honey and propolis activity and honey against bacteria isolated from diabetic foot ulcer lesion
Study Design: In vitro antimicrobial activities of honey and crude hexane and methanolic extract of Libyan propolis against bacteria isolated from diabetic foot ulcer lesion
Place and Duration of Study: Samples collected from patient in Tripoli Iben Nafees Hospital using disc and agar diffusion method.
Methodology: Disk diffusion method on groups of aerobic and anaerobic bacteria were obtained from diabetic foot lesion.
Results: The result showed that the percentage of aerobic bacteria isolated from diabetic lesion was about 74%, which include MRSA, E. coli, Ps. aeruginose, Citrobacter, Pantoea, Proteus, Staphylococcus epidermis, Enterobacter and Serratia bacteria. Whereas anaerobic bacteria reported about 26% of Clostridium, Bacteroides, and Lactobacillus Jensenii.
Conclusion: It was concluded that the honey and propolis extract had antibacterial activity against a different type of aerobic and anaerobic bacteria that were isolated from diabetic foot ulcer lesion.
Keywords: Diabetic foot, Propolis, methicillin resistant Staphylococcus aureus, E. coli, Ps. aeruginosae, Citrobacter, Pantoea, Proteus, Staph, Epidermis.