Production of Modified Rapid Serum Agglutination Antigen for Sero-diagnosis of Contagious Bovine Pleuropneumonia (CBPP)
Raghad A. H. Onsa *
Department of Mycoplasma, Central Veterinary Research Laboratories, Alamarat, Animal Resources Research Corporation, Khartoum, Sudan.
F. M. Hamid
Department of Mycoplasma, Central Veterinary Research Laboratories, Alamarat, Animal Resources Research Corporation, Khartoum, Sudan.
E. I. Elshafie
Department of Parasitology, Central Veterinary Research Laboratories, Alamarat, Animal Resources Research Corporation, Khartoum, Sudan.
E. A. Muna
Department of Bacteria, Central Veterinary Research Laboratories, Alamarat, Animal Resources Research Corporation, Khartoum, Sudan.
G. E. Mohammed
Department of Microbiology, Faculty of Veterinary Medicine, Sudan University of Science and Technology, Sudan.
*Author to whom correspondence should be addressed.
Abstract
Aims: This work was conducted to develop rapid serum agglutination test for Contagious Bovine Pleuropneumonia (CBPP) serodiagnosis.
Study Design: Production of two types of antigen, one from T1/44 reference strain of Mycoplasma mycoides sub sp. mycoides (Mmm) and the other from local strain (RH).
Place and Duration of Study: This study was carried out at Mycoplasma department Central Veterinary Research Lab (CVRL) in Sudan during 2016-2018.
Methodology: The antigens have been developed using standard methods and evaluated using standard reference serum, c.ELISA and Latex Agglutination test. Statistical analysis using Chi-square test and Kappa agreement were applied.
Results: The sensitivity of the developed stained antigen from T1/44 strain (SAT1) was 100% in comparison of 92% and 95% of SAT2 (RH local strain) and LAT, respectively, The developed SAT2 antigen revealed satisfied specificity results (70%), which is higher than SAT1 (31%) and LAT (59.6%).
Conclusion: From these results we found that SAT2 which is produced from local strain- proved to be more specific and highly sensitive. The SAT2 combines low cost and high specificity and easy applicable in field, without need for any specialist training or equipment.
Keywords: Contagious, bovine, Pleuropneumonia, Mycoplasma mycoides subsp mycoides (Mmm), competitive ELISA, latex agglutination test, sensitivity, specificity.