Socio-demographic and Obstetric Variations of T. gondii and HIV-1 Co-infection among Pregnant Women in Cameroon

Main Article Content

Lem Edith Abongwa
Alberic Signang
Sharon Tibi
Annabella Ngenwi


Aim: This study, aimed to identify the seroprevalence of T. gondii and HIV-1 co-infection in pregnant women in the Northwest Region of Cameroon.

Study Design:  This cross-sectional study was conducted among 606 pregnant women attending antenatal clinic in the Northwest region of Cameroon.

Place and Duration of Study: This study was carried out at the Bamenda Regional Hospital from May 2017 to December 2017.

Methodology: Venous blood samples were collected for the detection of anti-Toxoplasma antibodies using rapid test kits while HIV was determine using Alere Determine™HIV-1/2 test kit and OraQuick HIV 1/2 Rapid Antibody Test kit for confirmation. Data were analyzed using SPSS version 23 statistical package. P-value <0.05 was considered statistically significant.

Results: The mean (SD) age was 27.3 (5.3) years. The prevalence of T. gondii and HIV-1 was 139 (22.9%) and 70(11.6%) respectively, while that of T. gondii and HIV-1 co-infection was 31(5.1%). With the exception of age group and gestational age that was significant (p<0.05) for HIV, socio-demographic and obstetrical characteristics of T. gondii, HIV-1 and T. gondii and HIV-1 co-infection prevalence did not show any significant differences (p>0.05).

Conclusions: The high prevalence of T. gondii and HIV-1 co-infection seen in this study demonstrates the need for routine antenatal screening for both infections. In addition, data from this study will be useful in designing control and prevention strategies against these diseases. Furthermore, the result will also be used as baseline data for further research on T. gondii and HIV-1 co-infection.

Co-infection, human immunodeficiency virus, pregnant women, toxoplasmosis, Cameroon

Article Details

How to Cite
Abongwa, L., Signang, A., Tibi, S., & Ngenwi, A. (2019). Socio-demographic and Obstetric Variations of T. gondii and HIV-1 Co-infection among Pregnant Women in Cameroon. Journal of Advances in Microbiology, 16(3), 1-11.
Original Research Article


da Silva MG, Vinaud MC, de Castro AM. Prevalence of toxoplasmosis in pregnant women and vertical transmission of Toxoplasma gondii in patients from basic units of health from Gurupi, Tocantins, Brazil. 2012 to 2014. PLoS One. 2015; 10(11):e0141700.

Nguefack HL, Gwet H, Desmonde S, Oukem-Boyer OO, Nkenfou C, Téjiokem M, et al. Estimating mother-to-child HIV transmission rates in Cameroon in 2011: A computer simulation approach. BMC Infectious Diseases. 2015;16(1):11.

Dawit G, Shishay K. Epidemiology, public health impact and control methods of the most neglected parasite diseases in Ethiopia: A review. World J Med Sci. 2014; 10:94-102.

Frimpong C, Makasa M, Sitali L, Michelo C. Seroprevalence and determinants of toxoplasmosis in pregnant women attending antenatal clinic at the University Teaching Hospital, Lusaka, Zambia. BMC infectious diseases. 2017;17(1): 10.

Wam EC, Sama LF, Ali IM, Ebile WA, Aghangu LA, Tume CB. Seroprevalence of Toxoplasma gondii IgG and IgM antibodies and associated risk factors in women of child-bearing age in Njinikom, NW Cameroon. BMC research notes. 2016; 9(1):406.

Negero J, Yohannes M, Woldemichael K, Tegegne D. Seroprevalence and potential risk factors of T. gondii infection in pregnant women attending antenatal care at Bonga Hospital, Southwestern Ethiopia. International Journal of Infectious Diseases. 2017;57:44-9.

Yohanes T, Zerdo Z, Chufamo N. Seroprevalence and predictors of hepatitis B virus infection among pregnant women attending routine antenatal care in Arba Minch Hospital, South Ethiopia. Hepatitis research and treatment;2016.

Paul E, Kiwelu I, Mmbaga B, Nazareth R, Sabuni E, Maro A, et al. Toxoplasma gondii seroprevalence among pregnant women attending antenatal clinic in Northern Tanzania. Tropical Medicine and Health. 2018;46(1):39.

Nazari N, Bozorgomid A, Janbakhsh A, Bashiri F. Toxoplasma gondii and human immunodeficiency virus co-infection in western Iran: A cross-sectional study. Asian Pacific Journal of Tropical Medicine. 2018;11(1):58.

Njunda AL, Assob JC, Nsagha DS, Kamga HL, Nde PF, Yugah VC. Seroprevalence of Toxoplasma gondii infection among pregnant women in Cameroon. Journal of Public Health in Africa. 2011;2(2).

Simpore J, Savadogo A, Ilboudo D, Nadambega MC, Esposito M, Yara J, et al. Toxoplasma gondii, HCV, and HBV seroprevalence and co‐infection among HIV‐positive and‐negative pregnant women in Burkina Faso. Journal of Medical Virology. 2006;78(6):730-3.

Okwuzu JO, Odunukwe NN, Ezechi OC, Gbajabiamila TA, Musa AZ, Ezeobi PM, et al. Toxoplasma gondii infection in HIV/ AIDS: Prevalence and risk factors. African Journal of Clinical and Experimental Microbiology. 2014;15(2):97-102.

Shao ER, Ndazana SG, Chacha W, Masenga G, Tolbert S, Mosha D, et al. Sero-prevalence and factors associated with Toxoplasma gondii infection among pregnant women attending antenatal care in the Referral Hospital in Tanzania: Cross sectional study iMedPub Journals. Annals of Clinical and Laboratory Research. 2015; 3(2):17.

Fernandes MA, Batista GI, Carlos JD, Gomes IM, Azevedo KM, Setúbal S, Oliveira SA, et al. Toxoplasma gondii antibody profile in HIV-1-infected and uninfected pregnant women and the impact on congenital toxoplasmosis diagnosis in Rio de Janeiro, Brazil. Brazilian Journal of Infectious Diseases. 2012;16(2):170-174.

Wang ZD, Wang SC, Liu HH, Ma HY, Li ZY, Wei F, et al. Prevalence and burden of Toxoplasma gondii infection in HIV-infected people: A systematic review and meta-analysis. The Lancet HIV. 2017;4(4): e177-88.

de Dieu Anoubissi J, Gabriel EL, Nde CK, Fokam J, Tseuko DG, Messeh A, Moussa Y, Nkenfou CN, Bonono L, Billong SC, Nfetam JB. Factors associated with risk of HIV-infection among pregnant women in Cameroon: Evidence from the 2016 National Sentinel Surveillance Survey of HIV and syphilis. BioRxiv. 2018;482901.

Yang S, Yang C, Liao Q, Zhai W, Yu G, Xiao L, et al. Analysis of HIV prevalence among pregnant women in Liangshan Prefecture, China, from 2009 to 2015. PloS One. 2017;12(9):e0183418.

Kalua T, Barr BA, Van Oosterhout JJ, Mbori-Ngacha D, Schouten EJ, Gupta S, et al. Lessons learned from option B+ in the evolution toward "test and start" from Malawi, Cameroon, and the United Republic of Tanzania. JAIDS Journal of Acquired Immune Deficiency Syndromes. 2017 May 1;75: S43-50.

Kiptoo M, Mpoke S, Mueke J, Okoth F, Songok E. Survey on prevalence and risk factors on HIV-1 among pregnant women in North-Rift, Kenya: A hospital-based cross-sectional study conducted between 2005 and 2006. BMC international health and human rights. 2009;9(1):10.

Dionne-Odom J, Mbah R, Rembert NJ, Tancho S, Halle-Ekane GE, Enah C, et al. Hepatitis B, HIV, and syphilis sero-prevalence in pregnant women and blood donors in Cameroon. Infectious Diseases in Obstetrics and Gynecology; 2016.

Sama CB, Feteh VF, Tindong M, Tanyi JT, Bihle NM, Angwafo III FF. Prevalence of maternal HIV infection and knowledge on mother–to–child transmission of HIV and its prevention among antenatal care attendees in a rural area in northwest Cameroon. PloS One. 2017;12(2): e0172102.

Teto G, Tagny CT, Mbanya D, Fonsah JY, Fokam J, Nchindap E, et al. Gag P2/NC and pol genetic diversity, polymorphism, and drug resistance mutations in HIV-1 CRF02_AG-and non-CRF02_AG-infected patients in Yaounde, Cameroon. Scientific reports. 2017;7(1):14136.

Nayak AK, Jain MK, Dhivya S, Hota S. A study on the prevalence of HIV infection among pregnant women attending antenatal clinic in a tertiary care hospital, Cuttack, India. International Journal of Community Medicine and Public Health. 2017;4(5):1504-9.

Gelaye W, Kebede T, Hailu A. High prevalence of anti-toxoplasma antibodies and absence of Toxoplasma gondii infection risk factors among pregnant women attending routine antenatal care in two Hospitals of Addis Ababa, Ethiopia. International Journal of Infectious Diseases. 2015;34:41-5.

Patil VM, Moray AP, Patil SP. Ten years trend of HIV seroprevalence among Indian Pregnant women attending antenatal clinic at a tertiary hospital in Dhule, Maharashtra. India. IJRCOG. 2016;5(5):1514-9.

Smith J, Nalagoda F, Wawer MJ, Serwadda D, Sewankambo N, Konde-Lule J, et al. Education attainment as a predictor of HIV risk in rural Uganda: results from a population-based study. International Journal of STD & AIDS. 1999; 10(7):452-9.

Cong W, Dong XY, Meng QF, Zhou N, Wang XY, Huang SY, et al. Toxoplasma gondii infection in pregnant women: a seroprevalence and case-control study in Eastern China. BioMed Research Inter-national; 2015.

Awoke K, Nibret E, Munshea A. Sero-prevalence and associated risk factors of Toxoplasma gondii infection among pregnant women attending antenatal care at Felege Hiwot Referral Hospital, northwest Ethiopia. Asian Pacific Journal of Tropical Medicine. 2015;8(7):549-54.

Ndumbe PM, Andela A, Nkemnkeng-Asong J, Watonsi E, Nyambi P. Prevalence of infections affecting the child among pregnant women in Yaounde, Cameroon. Medical Microbiology and Immunology. 1992;181(3):127-30.

Yusuf AM, Yahaya S, Azeez-Akande O. Seroprevalence and risk factors of Toxoplasma gondii infection (Toxoplasmosis) among HIV seropositive pregnant women in a tertiary healthcare centre, Kano, Northern Nigeria. J. Med. Med. Sci. 2016;7:001-5.

Walle F, Kebede N, Tsegaye A, Kassa T. Seroprevalence and risk factors for Toxoplasmosis in HIV infected and non-infected individuals in Bahir Dar, Northwest Ethiopia. Parasites & vectors. 2013;6(1):15.

Kamal AM, Ahmed AK, Abdellatif MZ, Tawfik M, Hassan EE. Seropositivity of toxoplasmosis in pregnant women by ELISA at Minia University Hospital, Egypt. The Korean Journal of Parasitology. 2015; 53(5):605.

Chaudhry SA, Gad N, Koren G. Toxoplasmosis and pregnancy. Canadian Family Physician. 2014;60(4):334-6.

Elsheikha HM, Aboul-Dahab MA, Abdel AM, El-Sherbini ET. Prevalence and risk factors of Toxoplasma gondii antibodies in asymptomatic Egyptian blood donors. Journal of the Egyptian Society of Parasitology. 2009;39(Suppl1):351-61.

Alsammani MA. Sero-epidemiology and risk factors for Toxoplasma gondii among pregnant women in Arab and African countries. Journal of Parasitic Diseases. 2016;40(3):569-79.

Shimelis T, Tebeje M, Tadesse E, Tegbaru B, Terefe A. Seroprevalence of latent Toxoplasma gondii infection among HIV-infected and HIV-uninfected people in Addis Ababa, Ethiopia: A comparative cross-sectional study. BMC Research Notes. 2009;2(1):213.

Sitoe SP, Rafael B, Meireles LR, Andrade Jr HF, Thompson R. Preliminary report of HIV and Toxoplasma gondii occurrence in pregnant women from Mozambique. Revista do Instituto de Medicina Tropical de São Paulo. 2010;52(6):291-5.