Investigations on Bacteriological Quality of Tap Water Sources within the University of Port Harcourt

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H. O. Stanley
C. J. Ugboma
P. C. Uzoaru


In this study, the sanitary quality of tap water sources within the University of Port Harcourt was investigated in order to determine its suitability for drinking and other domestic applications. Eight composite samples of tap water were collected from Eight different locations within the three campuses of the University of Port Harcourt using 300 ml-capacity sterile containers. These locations included NDDC Hostel, Sports Hostel, Medical Hostel, NUH Hostel, Dan Etete Hostel, Delta A Hostel, Delta B Hostel and Choba Campus. After collection, water samples were taken to the laboratory for enumeration and identification of Total heterotrophic bacteria (THB), Total coliform (TC) and Faecal coliform (FC) using the membrane filtration method. Result showed that the average THB, TC and FC counts recorded in the tap water samples across the eight locations ranged from 12.4 CFU to 36.7 CFU, 4.3 CFU to 10.1 CFU and 2.8 CFU to 5.2 CFU per 100ml respectively. Bacterial isolates were identified as probably belonging to genera such as Bacillus spp., Klebsiella spp., Enterobacter spp., Staphylococcus spp., Proteus spp., Citrobacter spp., Serratia spp. and Escherichia spp., respectively. Samples from Delta B hostel and Choba campus recorded the lowest and highest THB, TC and FC counts respectively. According to WHO standard, the result of THB, TC and FC counts indicated that the tap water in all eight locations were above the limit acceptable for drinking but within acceptable limit for other domestic use such as bathing and washing.

Tap water sources, University of Port Harcourt, coli form bacteria.

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How to Cite
Stanley, H., Ugboma, C., & Uzoaru, P. (2019). Investigations on Bacteriological Quality of Tap Water Sources within the University of Port Harcourt. Journal of Advances in Microbiology, 16(3), 1-5.
Original Research Article


Shar AH, Kazi YF, Zardari M, Soomro IH. Enumeration of total and faecal coliform bacteria in drinking water of Khairpur City, Sindh, Pakistan. Bangladesh Journal of Microbiology. 2007;24(2):163-165.

Vahith RA, Sirajudeen J. Quantitative determination of total and faecal coliforms in groundwater between Tamil Nadu and Pondicherry States, India. Journal of Environmental Science and Pollution Research. 2016;2(1):57–59.

Adekunle IM, Adetunji MT, Gbadebo AM, Banjoko OB. Assessment of groundwater quality in a typical rural settlement in Southwest, Nigeria. International Journal of Environmental Research and Public Health. 2007;4(4):307-318.

Kisteman T, ClaBen T, Koch C, Dangendorf F, Fischeder R, Gebel J, Vacata V, Exner M. Microbial load of drinking water reservoir tributaries during extreme rainfall and runoff. Journal of Applied Environmental Microbiology. 2002; 68:2188-2197.

Lee DG, Kim SJ. Bacterial species in biofilms cultivated from the end of the Seoul water distribution system. Journal of Applied Microbiology. 2003;95:317- 324.

Leoni E, De Luca G, Legnan PP, Sacchetti R, Stampi S, Zanetti F. Legionella waterline colonization: Detection of Legionella species in domestic, hotel and hospital hot water systems. Journal Applied Microbiology. 2005;98:373-379.

Alonso JL, Soriano A, Carbajo O, Amoros I. Comparison and recovery of Escherichia coli and thermotolerant coliforms in water with a chromogenic medium incubated at 41 and 44°C. Journal Applied Environ-mental Microbiology. 1999;65:3746-3749.

Lisle JT, Smith JJ, Edwards DD, McFeters GA. Occurrence of microbial indicators and Clostridium perfringens in wastewater, water column samples, sediments, drinking water, and Weddell Seal faeces collected at McMurdo station Antarctica. Journal of Applied Environmental Microbiology. 2004; 70: 7269-7276.

Kaltenthaler EC, Drasar BS, Potter CW. The use of microbiology in study of hygiene behaviour. Faculty Press, Cambridge. 1996;88:35-43.

Anderson KL, Whitlock JE, Harwood VJ. Persistence and differential survival of faecal indicator in subtropical waters and sediments. Journal of Applied Environ-mental Microbiology. 2005;71:3041-3048.

Araujo RM, Puig A, Lasobras J, Lucena F, Jofre J. Phages of enteric bacteria in fresh water with different levels of faecal pollution. Journal of Applied Microbiology. 1997;82:281-286.

Ejaz M, Ahmed A. Physical, chemical and biological parameters in well waters of Karachi and their health impacts. Journal of Chemical Society of Pakistan. 2001;23: 263-267.

Kirschner AKT, Zechmeister TC, Kavka GG, Beiwl C, Herzig A, Mach RL, Farnleitner, AH. Integral strategy for evaluation of faecal indicator performance in bird-influenced saline inland water. Journal of Applied Environmental Micro-biology. 2004;70:7396-7403.

Abdulsalam I, Rafida A, Altabet A, Ramadan G. The presence of total coliform and faecal coliform bacteria in rainwater harvesting in Jefara district of Libya. International Conference on Ecology, Environment and Biological Science. 2012; 7(8):340-342.

Antony RM, Renuga FB. Microbiological analysis of drinking water quality of Ananthanar channel of Kanyakumari district, Tamil Nadu, India. Interdisciplinary Journal of Applied Science. 2012;7(2):42-48.

APHA. Standard methods for the examination of water and wastewater 21st ed. Washington, DC: American Public Health Association; 2005.

Holt JG, Krieg NR. Bergey’s manual of determinative bacteriology (9th Ed). The Williams and Wilkins Co., Baltimore; 1994.

Payus C, Nandini U. The presence of total coliform and faecal coliform in the private beach resort of Sabah. Borneo Science. 2014;34:1-5.