Optimization of Cellulose Production by Curvularia pallescens Isolated from Textile Effluent

Main Article Content

Suman Mehla
Soumana Datta


Introduction: Celluloses are important industrial enzymes and find application in several industrial processes. Effects of pH, temperature, incubation time, source of carbon and nitrogen were tested in submerged fermentation process in the production of cellulose by Curvularia pallescens isolated from textile effluent.

Aims: The present study was attempted in a fungus; Curvularia pallescens isolated from textile effluent for maximizing its production under optimal conditions in submerged fermentation by using inexpensive substrate wheat bran.

Study Design: The production medium was prepared in distilled water, supplemented with 4.5% wheat bran, 0.05% KCl, 0.2% KH2PO4, (carbon source), yeast extract (nitrogen source), maintained with pH of  5.5  and incubated at 28ºC for 120 h was found optimal for the production of cellulose.

Results: The test fungus achieved maximum FPA activity followed by cellobiohydrolase, endoglucanase and β-glucosidase activity at  46.76, 42.06, 26.94 and 3.56 U/ml respectively at pH 5.5  (Fig. 4). The temperature of 280C produced maximum cellulase activity. Highest activity recorded was of FPA (38.94 U/ml), followed by cellobiohydrolase (30.29 U/ml), endoglucanase (22.41 U/ml), and β-glucosidase (3.98 U/ml). The effect of process parameters such as the effect of temperature, pH and inoculum size was investigated. Maximum cellulase and xylanase having an enzyme activity of 694.45 and 931.25 IU, respectively, were produced at 30ºC incubation temperature.

Conclusion: The effect of process parameters such as effect of temperature, pH and inoculum size was also investigated. The production of primary metabolites by microorganisms is highly influenced by their growth, which is determined by the availability of the nutrients in the substrates.

Cellulase, Curvularia pallescens, textile effluent, submerged fermentation, wheat bran.

Article Details

How to Cite
Mehla, S., & Datta, S. (2020). Optimization of Cellulose Production by Curvularia pallescens Isolated from Textile Effluent. Journal of Advances in Microbiology, 20(1), 11-17. https://doi.org/10.9734/jamb/2020/v20i130205
Short communication


Kang SW, Park YS, Lee JS, Hong SI, Kim SW. Produciton of cellulases and hemicellulases by Aspergillus niger KK2 from lignocellulosic biomass. Bioresour. Technol. 2004;153-156.

Haltrich D, Nidetzky B, Kulbe KD, Steiner W, Zupancic S. Production of fungal xylanases. Bioresour. Technol. 1996;58: 137-161.

Hanif A, Yasmeen A, Rajoka MI. Induction, production, repression, and de-repression of exoglucanase synthesis in Aspergillus niger. Bioresource Technology. 2004; 94(3):311–319.

Graca MAS, Perria RCF. The ability of selected aquatic hypomycetes and terrestrial fungi to decompose leaves in freshwater. Sydowia.1995;47(2):167–179.

Mandels M, Andreotti R, Roche C. Measurment of saccharifying cellulase. In Enzymatic Conversion of Cellulosic Materials: Technology and Applications. Biotechnology & Bioengineering Symposium. 1976;6:21-33.

Rosenberger RF. In The Filamentous Fungi (eds Smith, J. E. and Berry, D. R.), Edward Arnold, London. 1975;2:328–342.

Garcia KO, Munoz AM, Perez V, Rand, Huitron VC. Mixed submerged fermentation with two filamentous fungi for cellulolytic and xylanolytic enzyme production. Appl. Biochem. Biotechnol. 2002;1105(14):98-100.

Chellapandi Paulchamy, Jani Abha Apurvabhai. Enhanced Endoglucanase Production by Soil Isolates of Fusarium sp. and Aspergillus sp. through Submerged Fermentation Process. Turkish Journal of Biochemistry–Turk J Biochem. 2009; 34(4);209–214.

Papinutti Leandro, Bernardo Lechner. Influence of the carbon source on the growth and lignocellulolytic enzyme production by Morchella esculenta strains. J Ind Microbiol Biotechnol. 2008;35:1715–1721.

Arora DS, Gill PK. Laccase production by some white rot fungi under different nutritional conditions. Bioresource Technol. 2000;73:283-285.

Gupta GS, Prasad G, Singh VH. Removal of chrome dye from aqueous solutions by mixed adsorbents: fly ash and coal, Water Res. 1990;24:45–50.

Li Y, Lin J, Meng D, Lu J, Gu G, Mao Z. Effect of pH, cultivation time and substrate concentration on the Endoxylanase production by Aspergillus awamori ZH-26 under submerged fermentation using central composite rotary design. Food. Technol. Biotechnol. 2006;44:473-477.

Reddy V, Reddy P, Pillay B, Singh S. The effect of aeration on the production of hemicellulases by Thermomyces lanuginosus SSBP in a 30 l bioreactor. Process Biochem. 2002;37:1221-1228.

Singh S, Pillay B, Dilsook V, Prior BA. Production and properties of hemicellulases by Thermomyces lanuginosus strain. J. Appl. Microbiol. 2000;88:975-982.

Acharya PB, Acharya DK, Modi HA. Optimization for cellulase production by Aspergillus niger using saw dust as substrate. African Journal of Biotechnology. 2008;7(22):4147-4152.

Ojumu TV, Solomon BO, Betiku E, Layokun SK and Amigun B. Cellulase production by Aspergillus flavus Linn isolate NSPR 101 fermented in sawdust, bagasse and corncob. Afr. J Biotechnol. 2003;2(6):150-152.