Main Article Content
Microflora from potato rhizosphere soil was isolated from different potato fields of Bangladesh. Seventeen soil samples were analyzed for the presence of microflora in selected potato field soils. Seven fungal species and one bacterium species were morphologically characterized using soil dilution and streak plate methods. The predominant fungi isolated including Alternaria sp., Aspergillus sp., Penicillium sp., Rhizopus sp., Bipolaris sp., Phytophthora sp., Fusarium sp. and one bacterium was identified as Ralstonia solanacearum. Individual colonies of fungi and bacteria were counted on Potato Dextrose Agar (PDA), V8 juice Agar and their presence in soil was compared in respect of different locations of potato fields. The occurrence of Phytophthora sp. was medium in Tongibari and lower in Singair Union, Sonargaon, Matlab Dakshin, Gobindaganj, Palashbari, Gopinathpur and Bagmara. The highest counts of R. solanacearum were found in Singair Union, Tongibari and Daudkandi and the lowest counts were made in Palashbari and Bagmara. This was the first reported examination of the microbial diversity of soil microflora in some selected potato fields of Bangladesh.
Singh K, Borana J, Srivastava S. Effect of thiram on root growth, root nodules and nitrogen fixation in Glycine max (L) merril by Bradyrhizobiam japonicom. Journal of Soil Biology and Ecology. 1999;19 :11-14.
Mc.Gill WB, Cannon KR, Robertson JA, Cook FD. The Role of Soil Microbial Biomass in Burned Japanese Red Pine Forest. Canadian Journal of Soil Science. 1980;66.
Manickam TS, Venkataraman CR. Influence of Fertilization and different tillage systems on soil micro flora. Madras Agricultural Journal. 1972;59:508-512.
Ainsworth GC, Bisby GR. Dictionary of the fungi. 8th ed. Wallingford: CABI. 1995;445.
Barbhuiya AR, Arunachalam A, Pandey HN, Arunachalam K, Khan ML, Nath PC. Dynamics of soil microbial biomass C, N and P in disturbed and undisturbed stands of a tropical wet-evergreen forest. European Journal of Soil Biology. 2004;40:113–121.
Field JA, Jong DE, Costa FG, Bont JAM. Screening for ligninolytic fungi applicable to the biodegradation of xenobiotics. Trends in Biotechnology. 1993;11(2):44-49.
Ali MS, Haque A. Potato for food security in SAARC Countries; SAARC Seed Congress and Fair 2011, Dhaka, Bangladesh; 2011.
Sunaina V, Kishore V, Shekhawat GS. Latent survival of Pseudomonas solanacearumin potato tubers and weeds. Journal of Plant Disease and Protection. 1889;96:361-364.
Yabuuchi E, Kosako Y, Yano I, Hotta H, Nishiuchi Y. Transfers of two Burkholderiu and on Alcaligenes species• to Ralstonia gen. Nov: Proposal of Ralstonia pickettii (Ralston, Palleroni and Duudroff 1973) Comb. Nov. Ralstonia solanacearum (Smith, 1896) Comb. Nov. and Ralstoniaeutropha (Davies 1969) Comb. Nov. Microbiology and Immunology. 1995; 36:1251-1275.
Elphinstone JG. The current bacterial wilt situation: A global overview. Pages 9-28 in: Bacterial Wilt: The Disease and the Ralstonia solanacearum Species Complex. C. Allen, P. Prior and A. C. Hayward, eds. American Phytopathological Society, St. Paul, MN; 2005.
Malik MT, Dasti AA, Khan SM. Mango decline disorders prevailing in Pakistan. Proceedings of International Conference on Mango and Date palm: Culture and Export, University of Agriculture, Faisalabad, Pakistan. 2005;53-60.
Razak AA, Bachman G, Farrag R. Activities of micro flora in soils of upper and lower Egypt. The African J.of Mycol. And Biotech.1999;7(1):1-19.
Adesemoye AO, Opere BO, Makinde SC. Microbial abattoir waste water and its contaminated soil in Lagos, Nigeria. African Jr. of Biotechnol.2006;5(20):1963-1968.
Watanabe T. Pictorial Atlas of Soil and Seed Fungi. Morphogenesis of cultured fungi and Key to species. Third Edition. CRC Press; 2010.
Buck, JD. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Applied and Environmental Microbiology. 1982;44(4):992-993.
Rohilla SK, Salar RK. Isolation and Characterization of Various Fungal Strains from Agricultural Soil Contaminated with Pesticides. Research Journal of Recent Sciences. 2012;1(ISC-2011):297-303.
Azaz AD. Isolation and Identification of Soil borne Fungi in Fields Irrigated by GAP in Harran Plain Using Two Isolation Methods. Turk. J. Bot. 2003;27:83-92.
Magnet MMH, Sarkar D, Zakaria A. Isolation And Identification Of Bacteria And Fungi From Soil Samples Of Different Industry Side In Dhaka City, Bangladesh. International Journal of Innovative Research &Development. 2013;2(8):38-39.
Gaddeyya G, Niharika PS, Bharathi P, Kumar PKR. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. Advances in Applied Science Research. 2012;3(4):2020-2026.
Durowade KA, Kolawole OM, Uddin RO, Enonbun KI. Isolation of Ascomycetous Fungi from a Tertiary Institution Campus Soil. J. Appl. Sci. Environ. Manage. 2008; 12(4):57-61.
Jasuja ND, Saxena R, Subhash C, Joshi SC. Isolation and identification of microorganism from polyhouse agriculture soil of Rajasthan. African Journal of Microbiology Research. 2013;7(41):4886-4891.
Nagrale DT, Gaikwad AP, Sharma L. Morphological and cultural characterization of Alternaria alternata (Fr.) Keissler blight of gerbera (Gerbera jamesonii H. Bolus ex J.D. Hook). Journal of Applied and Natural Science. 2013;5(1):171-178.
Tsao P, Ocana G. Selective Isolation of Species of Phytophthora from natural soils on an improved antibiotic medium. Nature. 1969;223:636-638.
Edinger-Marshall SB, Harpst S. Seasonal Variation in Isolation of Phytophthora from Wildland Soils. Humboldt State University, Arcata, CA. 2016;466-408.
Hayward AC. Characteristics of Psudomonas solanacearum. J. Appl. Bacteriol.1964;27(2):265-277.
Hayward AC. Some techniques of importance in the identification of Pseudomonas slanacearum. Pp. 137-142. In: Proc. Int. Plan Conf. Workshop Ecol. Control of Bacterial wilt caused by Pseudomonas solanacearum. Sequeira, L. and Kelman, A. (eds), North Carolina State University. Raleigh. 1976;166.
Mehan VK, McDonald D. Techniques for diagnosis of Pseudomonas solanacearum and for resistance screening against groundnut bacterial wilt. ICRISAT Technical Manual No. I.ICRISAT. Patencheru, Andhra Pradesh, India. 1995; 67.
Kelman A. The relationship of pathogenicity in Pseudomonas solanacearum to colony appreance on tetrazolium medium. Phytopathology. 1954;44:693-695.
Sharma MS, Tiwari KL. Isolation and identification of soil fungi from mahamera aniket, shivnath river durg. IOSR Journal of Environmental Science, Toxicology and Food Technology. 2015;1(5):24-26.
Toma FM, Abdulla NQF. Isolation, identification and seasonal distribution of soil borne fungi in different areas of Erbil Governorate. Journal of Advanced Laboratory Research in Biology. 2012;246-255.
Niharika PS, Gaddeyya G, Ratna PK. An investigation on soil mycoflora of different crop fields at Narasannapeta Mandal, Srikakulam District. International Research Journal of Environment Sciences. 2013; 2(9):38-44.
Vijayakumar R, Al-Aboody MS, Alturaiki W, Alsagaby SA, Sandle T. A study of airborne fungal allergens in sandstorm dust in Al-Zulfi, central region of Saudi Arabia. Journal of Environmental and Occupational Science. 2017;6(1):27-33.
Aminuzzaman FM, Jahan SN, Shammi J, Mitu AI, Liu XZ. Isolation and screening of fungi associated with eggs and females of root-knot nematodes and their biocontrol potential against Meloidogyne incognita in Bangladesh. Archives of Phytopathology and Plant Protection. 2018;51:288-308. DOI: 10.1080/03235408.2018.1472359.
Aminuzzaman FM, Xie HY, Duan WJ, Sun BD, Liu XZ. Isolation of nematophagous fungi from eggs and females of Meloidogyne spp. and evaluation of their biological control potential. Biocontrol Science and Technology. 2013;23(2):170-182.
Dey TK, Hossain M, Kadian MS, Hossain S, Bonierbale M, Mahmud AA, Prevalence, epidemiology and management of potato late blight in Bangladesh. Potato J. 2010;37(3-4):99-102.