Eco-Friendly Cinnamaldehyde Based Emulsion for Phytopathogenic Bacterial Growth Inhibitor

Main Article Content

Tahany G. M. Mohammed
A. F. Abd El- Rahman

Abstract

The formulation plays an essential role in achieving the successful delivery and biological activity of any plant protection products. This study aimed to develop a cinnamaldehyde water-based formulation (oil-in-water emulsion) via a high-shear stirring emulsification method. Cinnamaldehyde emulsion was successfully prepared and characterized using different physicochemical parameters (emulsion stability, persistent foaming, accelerated storage at 54°C for 2 weeks, and stability at 0°Cfor one week, as well as pH, surface tension, flash point, viscosity, and particle size distribution). Also, the antibacterial activity was verified in vitro against some important phytopathogenic bacteria; Erwinia amylovora, Pectobacterium aroidearum, Pseudomonas aeruginosa, and Ralstonia solanacearum using well diffusion method. In addition, the minimum inhibition concentration (MIC) was determined by the twofold dilution method. The results revealed that the prepared formulation showed good storage stability, exhibited non-Newtonian shear-thinning behavior and promising antibacterial activity. The inhibition zones against the tested phytopathogenic bacteria were ranged from 10.3 mm to 52.0 mm. MICs of the prepared formulation were 15.63, 31.25, 62.5, and 15.63 μl/ml against Erwinia amylovora, Pectobacterium aroidearum, Pseudomonas aeruginosa and Ralstonia solanacearum, respectively. Our results provide an environmentally friendly formulation with promising activity to control the agricultural crop disease.

Keywords:
Cinnamaldehyde, an oil-in-water emulsion, formulation, phytopathogenic bacteria

Article Details

How to Cite
Mohammed, T. G. M., & Rahman, A. F. A. E.-. (2020). Eco-Friendly Cinnamaldehyde Based Emulsion for Phytopathogenic Bacterial Growth Inhibitor. Journal of Advances in Microbiology, 20(10), 1-12. https://doi.org/10.9734/jamb/2020/v20i1030285
Section
Original Research Article

References

Benali T, Bouyahya A, Habbadi K, Zengin G, Khabbach A, Achbani EH, Hammani K. Chemical composition and antibacterial activity of the essential oil and extracts of Cistus ladaniferus subsp. ladanifer and Mentha suaveolens against phytopathogenic bacteria and their ecofriendly management of phytopathogenic bacteria. Biocatalysis and Agricultural Biotechnology. 2020;28:101696.

Kotan R, Cakir A, Ozer H, Kordali S, Cakmakci R, Dadasoglu F, Dikbas N, Aydin T, Kazaz C.Antibacterial effects of Origanum onites against phytopathogenic bacteria: Possible use of the extracts from protection of disease caused by some phytopathogenic bacteria. Scientia Horticulturae. 2014;172:210-220.

Kotan R, Cakir A, Dadasoglu F, Aydin T, Cakmakci R, Ozer H, Kordali S, Mete E, Dikbas N.Antibacterial activities of essential oils and extracts of Turkish Achillea, Satureja and Thymus species against plant pathogenic bacteria. Journal of the Science of Food and Agriculture, 2010;90(1):145-160.

Mansfield J, Genin S, Magori S, Citovsky V, Sriariyanum M, Ronald P, Dow M, Verdier V, Beer SV, Machado MA, Toth I, Salmond G, Foster GD.Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular Plant Pathology. 2012;13(6):614-629.

Kannan V, Bastas KK, Rajendran S. Scientific and Economic Impact of Plant Pathogenic Bacteria. 2015;369-392.

Pham DQ, Ba DT, Dao NT, Choi GJ, Vu TT, Kim J-C, Giang TPL, Vu HD, Le Dang Q. Antimicrobial efficacy of extracts and constituents fractionated from Rheum tanguticum Maxim. ex Balf. rhizomes against phytopathogenic fungi and bacteria. Industrial Crops and Products. 2017;108:442-450.

Isman MB. Plant essential oils for pest and disease management. Crop Protection. 2000;19(8):603-608.

Oliva MDLM, Carezzano ME, Giuliano M, Daghero J, Zygadlo J, Bogino P, Giordano W, Demo M. Antimicrobial activity of essential oils of Thymus vulgaris and Origanum vulgare on phytopathogenic strains isolated from soybean. Plant Biology. 2015;17(3):758-765.

Pedrotti C, Marcon ÂR, Delamare APL, Echeverrigaray S, Da Silva Ribeiro RT, Schwambach J. Alternative control of grape rots by essential oils of two Eucalyptus species. Journal of the Science of Food and Agriculture. 2019;99(14):6552-6561.

Ghalem BR. Essential Oils as Antimicrobial Agents Against Some Important Plant Pathogenic Bacteria and Fungi, in Plant-Microbe Interaction: An Approach to Sustainable Agriculture. Choudhary DK, Varma A, Tuteja N. Editors. Springer Singapore: Singapore. 2016;271-296.

Bajpai VK, Cho MJ, Kang SC. Control of Plant Pathogenic Bacteria of Xanthomonas spp. by the Essential Oil and Extracts of Metasequoia glyptostroboides Miki ex Hu In vitro and In vivo. Journal of Phytopathology. 2010;158(7‐8):479-486.

Mohammed T, El-Nahas S, Edris A.Formulation and evaluation of solvent-free microemulsions of lemongrass oil and citral as natural antifungal agent against some phytopathogenic fungi plant archives. 2019;19(2):2097-2107.

Bakhtiari S, Jafari S, Taheri JB, Kashi TSJ, Namazi Z, Iman M, Poorberafeyi M. The Effects of Cinnamaldehyde (Cinnamon Derivatives) and Nystatin on Candida Albicans and Candida Glabrata. Open access Macedonian journal of medical sciences. 2019;7:1067-1070. DOI: 10.3889/oamjms.2019.245.

Bevilacqua A, Corbo MR, Sinigaglia M.Combined effects of low ph and cinnamaldehyde on the inhibition of alicyclobacillus acidoterrestris spores in a laboratory medium. Journal of Food Processing and Preservation. 2008;32(5):839-852.

Doyle AA, Stephens JC. A review of cinnamaldehyde and its derivatives as antibacterial agents. Fitoterapia. 2019;139:104405.

Friedman M. Chemistry, Antimicrobial Mechanisms, and Antibiotic Activities of Cinnamaldehyde against Pathogenic Bacteria in Animal Feeds and Human Foods. J Agric Food Chem. 2017;65(48):10406-10423.

Yildiz ZI, Kilic ME, Durgun E, Uyar T. Molecular Encapsulation of Cinnamaldehyde within Cyclodextrin Inclusion Complex Electrospun Nanofibers: Fast-Dissolution, Enhanced Water Solubility, High Temperature Stability, and Antibacterial Activity of Cinnamaldehyde. Journal of Agricultural and Food Chemistry. 2019;67(40):11066-11076.

Mousavi F, Bojko B, Bessonneau V, Pawliszyn J. Cinnamaldehyde Characterization as an Antibacterial Agent toward E. coli Metabolic Profile Using 96-Blade Solid-Phase Microextraction Coupled to Liquid Chromatography–Mass Spectrometry. Journal of Proteome Research. 2016;15(3):963-975.

Mateen S, Rehman MT, Shahzad S, Naeem SS, Faizy AF, Khan AQ, Khan MS, Husain FM, Moin S. Anti-oxidant and anti-inflammatory effects of cinnamaldehyde and eugenol on mononuclear cells of rheumatoid arthritis patients. Eur J Pharmacol. 2019;852:14-24.

Gündel SDS,De Godoi, SN, Santos RCV, Da Silva JT, Leite, LBdM, Amaral AC, Ourique AF. In vivo antifungal activity of nanoemulsions containing eucalyptus or lemongrass essential oils in murine model of vulvovaginal candidiasis. Journal of Drug Delivery Science and Technology. 2020;57:101762.

Efrati R, Natan M, Pelah A, Haberer A, Banin E, Dotan A, Ophir A. The effect of polyethylene crystallinity and polarity on thermal stability and controlled release of essential oils in antimicrobial films. Journal of Applied Polymer Science. 2014;131(11).

Yazgan H. Investigation of antimicrobial properties of sage essential oil and its nanoemulsion as antimicrobial agent. LWT. 2020;130:109669.

Zhang X, Liu J. Effect of Arabic Gum and Xanthan Gum on the Stability of Pesticide in Water Emulsion. Journal of Agricultural and Food Chemistry. 2011;59(4):1308-1315.

Elekaei BehjatiH, Navvab Kashani M, Biggs MJ. Modelling of immiscible liquid-liquid systems by Smoothed Particle Hydrodynamics. Journal of Colloid and Interface Science. 2017;508:567-574.

Feng J, Chen Q, Wu X, Jafari SM, McClements DJ. Formulation of oil-in-water emulsions for pesticide applications: impact of surfactant type and concentration on physical stability. Environmental Science and Pollution Research. 2018;25(22):21742-21751.

Wang B, Tian H, Xiang D. Stabilizing the Oil-in-Water Emulsions Using the Mixtures of Dendrobium Officinale Polysaccharides and Gum Arabic or Propylene Glycol Alginate. Molecules. 2020;25(3):759.

Zhai X, Lin D, Liu D, Yang X. Emulsions stabilized by nanofibers from bacterial cellulose: New potential food-grade Pickering emulsions. Food Research International. 2018;103:12-20.

Silva BFB, Rodríguez-Abreu C, Vilanova N. Recent advances in multiple emulsions and their application as templates. Current Opinion in Colloid & Interface Science. 2016;25:98-108.

Kundu P, Kumar V, Mishra IM. Modeling the steady-shear rheological behavior of dilute to highly concentrated oil-in-water (o/w) emulsions: Effect of temperature, oil volume fraction and anionic surfactant concentration. Journal of Petroleum Science and Engineering. 2015;129:189-204.

Ferreira A, Vecino X, Ferreira D, Cruz JM, Moldes AB, Rodrigues LR. Novel cosmetic formulations containing a biosurfactant from Lactobacillus paracasei. Colloids Surf B Biointerfaces. 2017;155:522-529.

Chen K, Yu G, He F, Zhou Q, Xiao D, Li J, Feng Y. A pH-responsive emulsion stabilized by alginate-grafted anisotropic silica and its application in the controlled release of λ-cyhalothrin. Carbohydrate polymers. 2017;176:203-213.

Liu Y, Wei F, Wang Y, Zhu G. Studies on the formation of bifenthrin oil-in-water nano-emulsions prepared with mixed surfactants. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2011;389(1):90-96.

Chin C-P, Lan C-W, Wu H-S. Application of biodiesel as carrier for insecticide emulsifiable concentrate formulation. Journal of the Taiwan Institute of Chemical Engineers. 2012;43(4):578- 584.

Da Costa JT, Forim MR, Costa ES, De Souza JR, Mondego JM, Boiça Junior AL. Effects of different formulations of neem oil-based products on control Zabrotes subfasciatus (Boheman, 1833) (Coleoptera: Bruchidae) on beans. Journal of Stored Products Research. 2014;56:49-53.

Iqbal N, Kumar N, Saini MK, Dubey S, Agrawal A, Kumar J. Role of high shear mixing in improving stability and bio-efficacy of botanical oil in water formulation for early stage mosquito eradication. Heliyon. 2020;6(2):e03380.

Abd El-Rahman AF, Abolmaaty SM. Impact of climate change on the appearance of fire blight disease in a new area in Egypt.The Future Journal of Biology. 2020;3:37-48.

Abd El-Rahman AF, Balabel NM,Abd-El-Aziz RM. Isolation and identification of bacteria associated with Guava decline in Egypt. Journal of American Science. 2020;16(3):51-62.

Abd El-Rahman AF, Shaheen HA. Biological control of the brown rot of potato, Ralstonia solanacearum and effect of bacterization with antagonists on promotion of potato growth. Egyptian Journal of Biological Pest Control. 2016;26(4):733-739.

Mohammadi M,Moltmann E, Zeller W, Geider K. Characterisation of naturally occurring Erwinia amylovora strains lacking the common plasmid pEA29 and their detection with real-time PCR. European Journal of Plant Pathology. 2009;124(2):293-302.

Pastrik KH, Elphinstone JG, Pukall R. Sequence Analysis and Detection of Ralstonia solanacearum by Multiplex PCR Amplification of 16S–23S Ribosomal Intergenic Spacer Region with Internal Positive Control. European Journal of Plant Pathology. 2002;108:831-842.

Abd El-Rahman AF, Mohammed TGM. Soluble concentrate formulation of oxalic acid and N-Acetyl-L-cysteine: Potential of use in controlling Ralstonia solanacearum,J. of Plant Protection and Pathology, Mansoura Univ. 2020;11(9):427-434.

CIPAC, Collaborative International Pesticides Analytical Council. Disponível em: http://www.cipac.org />. Acesso em: 1 jun. 2016; 2016.

Du Z, Wang P. Gelatin Hydrolysate Hybrid Nanoparticles as Soft Edible Pickering Stabilizers for Oil-In-Water Emulsions. Molecules. 2020;25(2):393.

Daly SM, Sturge CR,Greenberg DE. Inhibition of Bacterial Growth by Peptide-Conjugated Morpholino Oligomers. Methods Mol Biol. 2017;1565:115-122.

Mohammad TGM, Abd El-Rahman AF. Environmentally friendly synthesis of silver nanoparticles using Moringa oleifera (Lam) leaf extract and their antibacterial activity against some important pathogenic bacteria. Mycopath. 2015;13(1):1 -6.

Bonev B, Hooper J, Parisot J. Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. The Journal of antimicrobial chemotherapy. 2008;61(6):1295-1301.

Keowmaneechai E, McClements DJ. Effect of CaCl2 and KCl on Physiochemical Properties of Model Nutritional Beverages Based on Whey Protein Stabilized Oil-in-Water Emulsions. Journal of Food Science. 2002;67(2):665-671.

WHO, Hardness in Drinking-water. Background document for development of WHO Guidelines for Drinking-water Quality, Washington, DC, USA; 2011.

Alvarado aguilar MC, Recalde coronelPC, Leal alvaradoDA, Villa sanchezFE, Tamayo alcivar R. Oil-in-water (o/w) emulsionable concentrate of ishpink (ocotea quixos) with thermodynamic stability. Revista Caatinga. 2019;32:590-598.

Karthik P, Ezhilarasi PN, Anandharamakrishnan C. Challenges associated in stability of food grade nanoemulsions. Crit Rev Food Sci Nutr. 2017;57(7):1435-1450.

Cao C, Song Y-Y, Zhou Z-L, Cao L-D, Li F-M, Huang Q-L. Effect of adhesion force on the height pesticide droplets bounce on impaction with cabbage leaf surfaces. Soft Matter. 2018;14(39):8030-8035.

Li Y, Feng Y, Yu G, Li J, Zhou Y, Liu Y. Preparation and characterization of oil-in-water emulsion based on eco-friendly emulsifiers. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2020;602:125024.

McClements DJ. Food emulsions : principles, practices, and techniques; 2016.

Zhong L, Oostrom M, Truex MJ, Vermeul VR, Szecsody JE. Rheological behavior of xanthan gum solution related to shear thinning fluid delivery for subsurface remediation. Journal of Hazardous Materials. 2013;244-245:160-170.

Xu J-L, Zhang J-C, Liu Y, Sun H-J, Wang J-H. Rheological properties of a polysaccharide from floral mushrooms cultivated in Huangshan Mountain. Carbohydrate Polymers. 2016;139:43-49.

Qiao L, Li Y, Chi Y, Ji Y, Gao Y, Hwang H, Aker WG, Wang P. Rheological properties, gelling behavior and texture characteristics of polysaccharide from Enteromorpha prolifera. Carbohydrate Polymers. 2016;136:1307-1314.

Song Y-R, Choi M-S, Choi G-W, Park I-K, Oh C-S. Antibacterial Activity of Cinnamaldehyde and Estragole Extracted from Plant Essential Oils against Pseudomonas syringae pv. actinidiae Causing Bacterial Canker Disease in Kiwifruit. The Plant Pathology Journal. 2016;32(4):363-370.

Di Pasqua R, Hoskins N, Betts G, Mauriello G. Changes in membrane fatty acids composition of microbial cells induced by addiction of thymol, carvacrol, limonene, cinnamaldehyde, and eugenol in the growing media. J Agric Food Chem. 2006;54(7):2745-9.

Gill AO, Holley RA. Mechanisms of bactericidal action of cinnamaldehyde against Listeria monocytogenes and of eugenol against L. monocytogenes and Lactobacillus sakei. Applied and environmental microbiology. 2004;70(10):5750-5755.

Gill AO, Holley RA. Inhibition of membrane boundATPases of Escherichia coli and Listeria monocytogenes by plant oilaromatics. 2006;111:170−174.

Gill AO, Holley RA. Disruption of Escherichia coli, Listeriamonocytogenes and Lactobacillus sakei cellular membranes by plant oilaromatics. Int. J. Food Microbiol. 2006;108:1−9.

Berthold-Pluta A., et al., Antibacterial activities of plant-derived compounds and essential oils against Cronobacter strains. European Food Research and Technology. 2019;245(5):1137- 1147.

Vasconcelos NG, Croda J, Simionatto S. Antibacterial mechanisms of cinnamon and its constituents: A review. Microb Pathog. 2018;120:198-203.