Determination of Anti-Pseudomonal Activities of Cocoa (Theobroma cacao) Pod Husk using Time-Kill-Test and Agar-Well Diffusion Techniques
Journal of Advances in Microbiology,
Aim: To determine the antimicrobial effectiveness of cocoa pod husk against P. aeruginosa.
Study Design: An experimental research
Place and Duration of Study: Department of Medical Microbiology, Olabisi Onabanjo University Teaching Hospital, Sagamu, Ogun State, Nigeria, between June 2015 and Sept. 2016.
Materials and Methods: Seventy-seven (77) isolates of P. aeruginosa obtained from three (3) teaching hospitals were involved in this study. Cocoa pod husk was processed into crude extract and its effectiveness against the isolates of P. aeruginosa were examined using agar well diffusion, time-kill test techniques and Anova.
Results: The percentage susceptibility of P. aeruginosa to Cocoa pod husk at 500 mg/mL and 250 mg/mL inhibited all the P. aeruginosa but only 14.29% were sensitive at a lower concentration of 31.25 mg/mL. Moreover, the comparison between the broth micro and macro dilution method of determining the MIC of cocoa pod husk against P. aeruginosa highlighted a significant decrease in the mean MIC value of the broth micro dilution method when compared with the broth macro dilution technique (T=13.519, P<0.05). The time kill test emphasized that P. aeruginosa was killed at a lower concentration of 62.5mg/ml at 150mins of introduction in to the Cocoa pod husk suspension.
Conclusion: This study revealed that the Cocoa pod husk possesses antibacterial properties. An increase in the concentration of cocoa pod husk increased its antibacterial activity against Pseudomonas aeruginosa. Moreover, the broth micro dilution technique is sensitive for determining the anti-pseudomonal activity.
- Pseudomonas aeruginosa
- cocoa pod husk
- agar well diffusion
- time-kill test.
How to Cite
Rehman A, Wayne M, Iain L. Mechanisms of ciprofloxacin resistance in Pseudomonas aeruginosa: new approaches to an old problem. J Med Microbiol 2019;68:1–10.
Lambert P. Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. J R Soc Med 2002;95:22-6.
Alaa A. Pseudomonas aeruginosa Pathogenesis and Pathogenic Mechanisms. I N Biology. 2015;7:44- 67.
Fagbohun E, Lawal O. In vitro evaluation of the antibacterial activity of crude extract from Phytophthora palmivora –Infected cocoa Pods on selected antibiotics resistant bacteria of clinical origin. NYSJ 2014;7:10.
Ajibesin K. Dacryodes edulis (G. Don) H.J. Lam: A review on its medicinal, phytochemical and economical properties. Res. J Med. Plant 2011;5:32-41.
Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: Past history and future perspective. J Herbmed Pharmacol 2018; 7:1-7.
Borchers A, Keen C, Hannum S. Gershwin M. Cocoa and chocolate: composition, bioavailability and health implications. J Med Food, 2000;3:77-103.
Santos R, Oliveira D, Sodré G, Gosmann G, Brende M, Pungartnik C. Antimicrobial activity of fermented Theobroma cacao pod husk extract. Genet Mol Res. 2014;13:7725-35.
Azila A, Azrina A, Amin I, Puziah H. Nur A. Antioxidant Properties of Cocoa Pods and Shells. Malaysian Cocoa Journal 2014;8:49-56.
Campos-Vega R, Karen H. Dave B. Cocoa (Theobroma cacao L.) pod husk: Renewable source of bioactive compounds. Trends in Food Science & Technology. 2018;81:172–84.
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