Isolation, Identification and Screening of Lipase Producing Fungi from the Soil Environment of Ilorin Metropolis
Journal of Advances in Microbiology,
Page 25-30
DOI:
10.9734/jamb/2022/v22i930485
Abstract
This investigation was carried out to isolate, identify and screen for lipase producing fungal species present in the soil environment of Ilorin metropolis. Soil samples of approximately 200g each were collected randomly from eight different locations within the Ilorin metropolis for the investigation. Potato Dextrose Agar was used for the isolation of the fungal species by pour plate method. Six fungal species, Penicillium spp, Acremoniumspp, Mucors pp, Rhizopus stolonifer, Aspergillus nigerand Aspergillus flavuswere isolated and screened for their ability to produce lipases on tween-20 and phenol red agar. The results obtained for lipase production on tween-80 and phenol red after 5 days of incubation showed that four isolates were positive for lipase production which was indicated by diameter zone of clearance and visible precipitate of calcium monolaurate due to the deposition of calcium crystal. The result further revealed that Aspergillus niger had the highest lipase producing ability (having a diameter zone of clearance of 14 ± 0.05 mm), followed by Rhizopus stolonifer (having 10 ± 0.05 mm). Aspergillus flavus and Mucor sp had 6 ± 0.03 mm, 6± 0.01 mm respectively. Acremonium sp. and Penicillium sp. had no zone of clearance. These results demonstrate the presence of lipase producing fungi in the soil environment of Ilorin metropolis,Kwara State, and these can be harnessed locally for large scale production of the enzyme which is of value commercially in the production of leather, detergent,textiles and also as constituents of some special diets and pharmaceuticals.
Keywords:
- Lipase
- soil
- fungi
- Ilorin
- Nigeria
How to Cite
References
Dabhi BK, Vyas RV, Shelat HN. Use of banana waste for the production of cellulolytic enzymes under solid substrate fermentation using bacterial consortium. International Journal of current Microbiology and Applied Science. 2014; 3(1):1–9.
Patel GB, Shah KR. Isolation, screening and identification of Lipase producing fungi from cotton seed soapstock. Indian Journal of Science and Technology. 2020; 13(36):3762-3771.
Roy M, Kumar R, Ramteke A. Sit N. Identification of lipase producing fungus isolated from dairy waste contaminated soil and optimization of culture conditions for Lipase production by the Isolated fungus. Journal of Microbiology, Biotechnology and Food Sciences. 2018;8(1):698–704.
Verma N, Thakur S, Bhatt AK. Microbial lipases: Industrial applications and properties (a review). International Research Journal of Biological Sciences. 2012;1(8):88–92.
Angelin J, Kavitha M. Extremophilic fungal lipases: Screening, purification, assay, and applications. In Extremophilic Fungi . Springer, Singapore. 2022;395-438.
Farias M, Valoni E, Castro A, Coelho MA. Lipase production by yarrowialipolytica in solid state fermentation using different agro industrial residues. Chemical engineering transactions. 2014;38:301–306.
Iftikhar T, Niaz M. Zia MA, Haq IU. Production of extracellular lipases by Rhizopusoligosporus in a stirred fermentor. Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. 2010;41(4):1124–1132.
Producing Fungi From the Soil Environment of Keffi Metropolis. Nigerian Journal of Microbiology. 31(1):3630-3635.
Sharma R, Chisti Y, Banerjee UC. Production, purification, characterization, and applications of lipases. Biotechnology Advances. 2001;19(8):627–662.
Hills G, Degussa AG. Industrial use of lipases to produce fatty acid esters. European Journal of Lipid Science and Technology. 2003;105:601–607.
Hellen T, Oliveria DD. A review on microbial lipase production. Food Bioprocess Technology. 2009;1935-5130.
Iftikhar MU, Sadarnac D, Karimi C. Input filter damping design for control loop stability of DC-DC converters. In 2007 IEEE International Symposium on Industrial Electronics. 2007;353-358. IEEE.
Nwuche CO, Ogbonna JC. Isolation of lipase producing fungi from palm oil mill effluent (POME) dump sites at Nsukka. Brazilian Archives of Biology and Technology. 2011;54:113-116.
White Bread AM, Lefroy RDB, Blair GC. Changes in physical properties and soil organic fractions with a cropping on a red brown earth soil. Australian society of Agronomy. 1996;12:23-29.
Kome GK, Enang R.K, Yerima BPK, Lontsi MGR. Models relating soil pH measurements in H2O, KCl and CaCl2 for volcanic ash soils of Cameroon. Geoderma Regional. 2018;14:e00185.
Crotty F, McCalman H, Powell H, Buckingham S, Marley C. Should farmers apply fertilizer according to when their daffodils are in flower? Utilizing a “farmer‐science” approach to understanding the impact of soil temperature on spring N fertilizer application in Wales. Soil use and management. 2019;35(1):169-176.
Fatima S, Rasool A, Sajjad N, Bhat EA, Hanafiah MM, Mahboob M. Analysis and evaluation of penicillin production by using soil fungi. Biocatalysis and Agricultural Biotechnology. 2019;21;101330.
Leonardi M, Furtado ANM, Comandini O. Geml J, Rinaldi AC. Halimium as an ectomycorrhizalsymbiont: new records and an appreciation of known fungal diversity. Mycological Progress. 2020;19(12):1495-1509.
Gopinath SCB, Anbu P, Hilda A. Extracellular enzymatic activity in fungi isolated from oil rich environment s.The Mycological Society of Japan and Springer verlag Tokyo. Mycoscience. 2005;46:119-126.
Makut MD, Bemgba US. Isolation and Identification of Lipase; 2017.
Davranov K. Microbial lipases in biotechnology. Prikladnaia Biokhimiia i Mikrobiologiia. 1994;30(4):527-534.
Mueller S, Ahmed KZ, Singh A, Davis AK, Mukhopadyay S, Swaminathan M, Mano Y, Wang Y, Wong J, Bharathi S, Moghadam HF. Design of high efficiency integrated voltage regulators with embedded magnetic core inductors. In 2016 IEEE 66th Electronic Components and Technology Conference (ECTC). 2016;566-573. IEEE.
-
Abstract View: 79 times
PDF Download: 37 times
