Antibiotic Resistance Profile of Escherichia coli and Klebsiella spp Isolated from Post-weaned Piglet Faeces under Penicillin-streptomycin Combination Therapy (Penstrep®) in Abidjan, Côte d’Ivoire
A. N. T. Koné *
Department of Food Science and Technology, Laboratory of Biotechnology and Microbiology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire.
N. K. N’gbesso
Environment Department, Ocean Research Center, Abidjan, Côte d'Ivoire.
B. K. Guédé
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
M. B. Ouattara
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
F. K. Konan
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
A. Dadié
Department of Food Science and Technology, Laboratory of Biotechnology and Microbiology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire.
N. K. Guessennd
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire and Department of Medical Sciences, Laboratory of Bacteriology-Virology, Félix Houphouet Boigny University, Abidjan, Côte d'Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Aim: The current study was aimed at evaluating the antibiotic resistance profile of Escherichia coli and Klebsiella spp isolated from post-weaned piglets treated with penicillin-streptomycin combination therapy.
Study Design: Bacteriological study.
Place and Duration of Study: Laboratory of the National Reference Center for antibiotics at Institut Pasteur Côte d’Ivoire, between March 2018 and June 2018.
Methodology: Escherichia coli and Klebsiella species were isolated from post weaned piglets stool on Mac Conkey medium added up separately with penicillin, streptomycin and combined penicillin-streptomycin and identified. Antibiotic susceptibility test was performed using disk diffusion method on Müller-Hinton agar.
Results: Escherichia coli and Klebsiella species resistance to penicillin, streptomycin and combined penicillin-streptomycin evaluated, respectively, reached 80.5% (Day 0) to 92.0% (Day 4); 17.0% (Day 0) to 39.9% (Day 4) and 31.3% (Day 0) to 70.9% (Day 4) for piglets treated with Penstrep®. In addition, antibiotic susceptibility test carried out for Escherichia coli and Klebsiella spp strains isolated from piglets treated with Penstrep® revealed resistance to amoxicillin for each bacterial species at a rate of 86.0% and 89.0%. Furthermore, control piglets showed lesser resistance to streptomycin (E.coli 31.0% and Klebsiella spp 38.0%) than those treated with the combination therapy Penstrep® (E. coli 73.0% and Klebsiella spp 48.0%). As for netilmicin, imipenem and colistin, no resistance was detected for treated piglets as well as untreated ones.
Conclusion: The combination therapy with Penstrep® has increased Escherichia coli and Klebsiella species resistance to antibiotics tested in the current study.
Keywords: Escherichia coli, Klebsiella spp, penicillin, streptomycin, antibiotic resistance