Evaluation of Different Genotypes and Genetic Virulence in Staphylococcus aureus Isolates from Clinical Samples
D. O. Ajayi
University Health Centre, Bamidele Olumilua University of Education, Science and Technology, Ikere-Ekiti, Ekiti State, Nigeria and Department of Chemical Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
A. A. Onasanya
Department of Chemical Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.
G. O. Daramola *
Department of Medical Microbiology and Parasitology, Ekiti State University, Teaching Hospital, Ado-Ekiti, Ekiti State, Nigeria and Department of Pure Sciences, National Open University of Nigeria, Ado-Ekiti Study Centre, Ado-Ekiti, Ekiti State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Infection with Staphylococcus aureus has been considered a major problem in hospitals. The clinical importance of S. aureus isattributed to notable virulence factors and genetic diversity. The objective of this study therefore was to investigate the distribution of S, aureus virulence gene anddifferent genotypesin some isolates obtained from clinical samples from Ekiti State University Teaching Hospital, Ado Ekiti. A total of 25 isolates were screened for the presence of 16SrRNA,GeIE and asaIvirulence genes using Polymerase Chain Reaction (PCR).Virulence genes 16SrRNA, gelE, asaI) formed clusters in S. aureus isolates used in this study. 16SrRNA was absence in 5 of the isolates and only present in 21 of the isolates. GeIE virulence gene was absence in 2 of the isolates and present in 23 of the isolates. AsaI virulence gene was absence in 7 of the isolates and present in 18 of the isolates. Virulence diversity was observed among isolates. Which could be used as a guide to the pathogenicity of individual isolates and hence control spread of infection.The genetic typing was carried out by Random Amplified Polymorphic DNA (RAPD-PCR) using OPBO8, OPHO4, OPH03, OPBO5, and OPT12 Primers. Also there exist different genotypes among the S. aureus isolates used in this study revealing high level of genetic diversity occurrence among S. aureus isolates. The DNA fingerprint revealed high genetic diversity among isolates irrespective of their sources. Further work could be done considering the antibiotic resistant gene and also sequencing of virulence gene clusters peculiar to S. aureus pathogens.
Keywords: Gene, virulence, staphyloccus aureus, invasiveness, genetic diversity.