Detection of Resistance Integron in Escherichia coli of Porcine Origin Producing Extended-spectrum Beta-lactamase in Abidjan, Côte d'Ivoire
I. K. Kouadio *
Laboratory of Microbiology and Molecular Biology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire
N. Guessennd
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
A. Dadié
Laboratory of Microbiology and Molecular Biology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire
V. Gbonon
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
E. Tahou
Laboratory of Genetics, Department of Biosciences, Félix Houphouet – Boigny University, Abidjan, Côte d'Ivoire
S. Kpoda
Laboratory of Applied and Nutritional Sciences, Ouaga I Professor Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
M. Dosso
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
*Author to whom correspondence should be addressed.
Abstract
Aims: Aim of the study was to detect resistance integrons involved in multidrug resistance phenotypes in Escherichia coli strains of porcine origin producing extended-spectrum beta-lactamases (ESBL).
Study Design: Genotypic study.
Place and Duration of Study: National Reference Center for Antibiotics and Molecular Biology Platform of Pasteur Institute of Côte d'Ivoire, between June 2017 at July 2017.
Methodology: Thirty-five (35) Escherichia coli strains of porcine origin producing extended-spectrum beta-lactamases were collected for study. The strains were analyzed using an antibiotic susceptibility test according to the diffusion method in agar medium. The research of class 1, 2 and 3 resistance integrons was performed using the conventional PCR method.
Results: 25 strains (71%) of E. coli producing ESBL harboured class 1 resistance integrons. None of the isolates carried class 2 and 3 resistance integrons. The strains harbouring resistance integrons were more resistant to amoxicillin, ceftriaxone, cefotaxime, tetracycline with a much higher rate of resistance (71%) compared to integron negative isolates (31%). The resistance to kanamycin and cotrimoxazole were 60% in integron positive isolates. Concerning integron negative isolates, the resistance to kanamycin was 11% and cotrimoxazole 20%.
Conclusion: The integron positive isolates is one of the major causes of resistance gene dissemination. This represents a risk for public health that must challenge the public authorities on the reasoned use of antibiotics in animal production chains.
Keywords: E. coli, extended-spectrum beta-lactamase (ESBL), antibiotic, piglets, integron resistance