Multi-Drug Resistant Campylobacter jejuni of Poultry Origin in Ado-Ekiti, Nigeria

O. O. Jegede

Ekiti State Ministry of Health, Ado-Ekiti, Ekiti State, Nigeria.

A. O. Ajayi

Department of Microbiology, Federal University, Oye-Ekiti, Ekiti State, Nigeria.

G. O. Daramola *

Department of Medical Microbiology and Parasitology, Ekiti State University Teaching Hospital, Ado-Ekiti, Ekiti State, Nigeria and Department of Pure Sciences, National Open University of Nigeria, Ado-Ekiti Study Centre, Ado-Ekiti, Ekiti State, Nigeria.

A. Ojo

Ekiti State College of Health Science and Technology, Ijero-Ekiti, Ekiti State, Nigeria.

A. Egbebi

Department of Medical Laboratory Science, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.

O. O. Ogunfolakan

Department of Medical Laboratory Science, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.

A. O. Ojerinde

Department of Medical Laboratory Science, Federal University, Oye-Ekiti, Ekiti State, Nigeria.

E. M. Aluko

Ministry of Health and Human Services, Secretariat Complex, Ado Ekiti, Ekiti State, Nigeria.

*Author to whom correspondence should be addressed.


The occurrence of Campylobacter jejeni in the large intestine of domestic fowls in Ado-Ekiti was assessed using standard microbiological procedures. One hundred faecal swabs were inoculated into modified cefoperazone charcoal deoxycholate agar (mCCDA). Twenty-seven isolates of Campylobacter jejeni were recovered from the birds. Biochemical identification of the isolates was carried out using oxidase and catalase tests. Antibiotic susceptibility test was carried out using standard disc diffusion method as specified by the Clinical Laboratory Standard Institute (CLSI), to the following antibiotics; amoxicillin, cefoperazone, ceftazidime, aztreonam, ceftriaxone, pefloxacin, ciprofloxacin, levofloxacin, enrofloxacin and norfloxacin. The pattern of resistance was as follows; Amoxicillin (66.7%), cefoperazone (48.1%), ceftazidime (66.7%), aztreonam (40.7%), ceftriaxone (74.15), pefloxacin (51.9%), ciprofloxacin (33.3%), levofloxacin (40.7%), enrofloxacin (22.25) and norfloxacin (59.3%). Twenty-three different multiple resistance pattern were observed among the isolates. The high level resistance observed in this study poses significant health risk to the general public, a synergistic collaboration is therefore suggested between public health policy-makers and researchers to curb this ugly trend.

Keywords: Gene, virulence, Campylobacter jejuni, invasiveness, genetic diversity

How to Cite

Jegede, O. O., Ajayi, A. O., Daramola, G. O., Ojo, A., Egbebi, A., Ogunfolakan, O. O., Ojerinde, A. O., & Aluko, E. M. (2022). Multi-Drug Resistant Campylobacter jejuni of Poultry Origin in Ado-Ekiti, Nigeria. Journal of Advances in Microbiology, 22(11), 107–114.


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Corry JEL, Atabay HI, Forsythe SJ, Mansfield LP. Culture media for the isolation of campylobacters, helicobacters and arcobacters, In: Corry JEL, Curtis GDW, Baird RM. Eds. Handbook of Culture Media for Food Microbiology. 2nd edn. Elsevier Sci. Amsterdam. 2003;271-315.

Van der walt ML. Campylobacter jejuni infection, in: infectious diseases livestock, 2nd Ed, edited by J. A. W. Coetzer and R. C. Tustin. Cape town: Oxford University Press. 2004;1479-1483.

Ryan KJ, Ray CG. (editors). Sherris Medical Microbiology (4th ed.). McGraw Hill. 2004;378–80.

Fujimoto S, Allos BM, Misawa N, Patton CM, Blaser MJ. Restriction fragment length polymorphism analysis and random amplified polymorphic DNA analysis of Campylobacter jejuni strains isolated from patients with Guillian-Barre syndrome. Journal of Infectious Disease. 1997;176: 1105-1108.

Gaudreau C, Michaud S. Cluster of erythromycin- and ciprofloxacin-resistant Campylobacter jejuni subsp. jejuni from 1999 to 2001 in men who have sex with men, Quebec, Canada. Clinical Infection Diseases. 2003;37:131–136.

Luber P, Wagner J, Hahn H, Bartelt E. Antimicrobial resistance in Campylobacter jejuni and Campylobacter coli strains isolated in 1991 and 2001–2002 from poultry and humans in Berlin, Germany. Antimicrobial Agents Chemotheraphy. 2003;47:3825–3830.

Engberg J, Aarestrup FM, Taylor DE, Gerner-Smidt P, Nachamkin I. Quinolone and macrolides resistance in Campylobacter jejuni and Campylobacter coli: resistance mechanisms and trends in human isolates. Emergence Infection Diseases. 2001;7:24–34.

Altekruse SF, Stern NJ, Swerdlow DL. Campylobacter jejuni – An Emerging Foodborne Pathogen. Emerging Infectious Diseases. 1999;5(1):28-35.

Black RE, Levine MM, Clements ML, Hughes TP, Blaser MJ. Experimental Campylobacter jejuni infection in humans. Journal of Infectious Diseases. 1988; 157:472-479.

Boucher SN, Slater ER, chamberlain AH. L, Adams MR. Production and viability of coccoid forms of Campylobacter jejuni, Journal of Applied Bacteriology. 1994; 77:303-307.

Broman T, Palmgren H, Bergstrom S, Sellin M, Waldenstrom J, Chaveerach P, Keuzenkamp DA, Urlings HA, Lipman LJA, van Knapen F. In vitro study on the effect of organic acids on Campylobacter jejuni/coli populations in mixtures of water and feed. Poult. Sci. 2002;81:621–628.

Corry JEL, Post DE, Colin P, Laisney MJ. Culture media for the isolation of Campylobacters. International Journal of Food Microbiology. 1995;26:43-76.

Danielsson-Tham ML, Olsen B. Campylobacter jejuni in Black-Headed Gulls (Larus ridibundus): Prevalence, Genotypes, and Influence on C. jejuni Epidemiology; 2002.

Friedman CR, Niemann J, Wegener HC, Tauxe RV. Epidemiology of Campylobacter jejuni infections in the United States and other industrialised nations. Campylobacter (NachampkinI & Blaser, M. J, eds). 2000;121–138.

Geneva: WHO. World Health Organization. World Health Report 2002. Reducing Risks, Promoting Healthy Life; 2002.

Griffiths PL. Morphological change of Campylobacter jejuni growing in liquid culture. Letters in Applied Microbiology. 1993;17:152-155.

Hald B, et al. Flies and campylobacter infection of broiler flocks. Emerg. Infect. Dis. 2004; 10:1490–1492.

Humphrey, Tom et al. O'Brien S, Madsen M. Campylobacters as zoonotic pathogens: A food production perspective . International Journal of Food Microbiology. 2007;117(3):237–57.

Humprey T, O’Brien S, Madsen M. Campylobacters as zoonotic influence on C. jejuni epidemiology. Journal Clinical Microbiology. 2007;40:4594–4602.

Jassim SS, Malik A, Aldridge A. Small bowel perforation: An unusual cause. Grand Rounds. 2011;11:17–19.

Oriye O. The impact of urban expansion on the land use types of Ado Ekiti, Nigeria, Aribisala JO, Jemiriye, T.F and Adewole S.O. (eds.) University of Ado Ekiti. 2008;50-51.

Yildirim M, Ustanbulluoolu E, Ayvali B. Prevalence and Antibiotic Susceptibility of Thermophilic Campylobacter Species in Broiler Chickens. Turk. J. Vetenary of Animal Science. 2005;29:655-660.

McMahon DJ, Mahmood F. Endemic campylobacter in south auckland. CDNZ. 1993;93:70-72.

Moore JE, et al. Campylobacter. Vet Res. 2005;36(3):351–82.

Parkhill J, Wren BW, Mungall K. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature. 2000;403:665–668.

Pathogens: A food production perspective. International Journal of Food Microbiology. 117:237-257.

Pearson AD, Greenwood M, Healing TD, Rollins D, Shahamat M, Donaldson J. Colonisation of Broiler chickens by waterborne Campylobacter jejuni. Applied Environmental Microbiology. 1993;59:987-986.

Perera VN, et al. Molecular mimicry in Campylobacter jejuni:role of the lipo-oligosaccharide core oligosaccharide in inducing anti-ganglioside antibodies. FEMS Immunology Medical Microbiology. 2007;50:27-36.

Prescott JF. Campylobacter in: Diagnostic procedures in veterinary Bacteriology and Mycology, 5th Ed, edited by G. R. carter and J. R. cole. San diego, California: Academic Press. 1990;6:61-75.

Saenz Y, Zarazaga M, Lantero M, Gastanares M, Bacuero F, Torres C. Antibiotic resistance in Campylobacter strains isolated from animals, foods and humans in Spain in 1997–1998. Antimicrobial Agents Chemotheraphy. 2000;44:267–271.

Simbert RM. The genus campylobacter. Annual Review of Microbiology. 1978; 32:673

Skirrow MB. Campylobacter enteritis. International Journal of Food Microbiology. 1991;12:9-16

Takkinen J, Ammon A, Robstad O, Breuer T, Campylobacter lGdts. Etude europe´enne sur la surveillance et le diagnostic de Campylobacter (2001). European Surveillance. 2003;8:207–213.

Vandamme P, Dewhist EE, Paster BJ. Genus I. Campylobacter sebald and Veron 1963, 907AL. In Bergeys manual of systematic Bacteriology. 2005;2(part C):1147-1160.

Kakoyiannis CK, Winter PJ, Marshall RB. The relationship between intestinal Campylobacter species isolated from animals and humans as determined by BRENDA. Epidemiology Infection. 1998; 100:379–387.

Ketley JM. Pathogens of enteric infection by campylobacter. Microbiology. 1997; 143:5-21.

Lastovica AJ. Emerging Campylobacter spp. the tip of the ice berg. Clinical Microiological Newsletter. 2006;28:49-56.

Lazaro B, Carcamo J, Audicana A, Perales I, Fernandez-Astorga A. Viability and DNA maintenance in nonculturablespiral C. jejuni cells after long-term exposure to low temperatures. Applied and Environmental Microbiology. 1999;65:4677-4681.

Lévesque B, Brousseau P, Bernier F, Dewailly É, Joly J. Study of the bacterial content of ring-billed gull droppings in relation to recreational water quality. Water Research. 2000;34:1089-1096.

McDermott PF, Bodeis SM, English LL, White DG, Walker RD, Zhao S, Simjee S, Wagner DD. Ciprofloxacin resistance in Campylobacter jejuni evolves rapidly in chickens treated with fluoroquinolones. Journal of Infectious Disease. 2002;185: 837–840.

Medema GJ, Schets FM, Vanne de Giessen AW, Harelaar AH. Lack of colonisation of one day-old chicks by viable non culturable Campylobacter jejuni. 1992;72:512-516

Miller WG, Wang G, Fontanoz S, Lastovica AJ. Extended multilocus sequence typing system for Campylobacter coli, C. lari, C. upsaliensis, and C. helveticus. Journal of Clinical Microbiology. 2005;43:2315–2329.

Moore JE, Barton MD, Blair IS. The epidemiology of antibiotic resistance in Campylobacter. Microbes of Infection. 2006;8:1955–1966.