Effect of Food Matrix, Cooking and Preservation Modalities on the Outgrowth of Bacillus cereus Spores in Some Starchy Foods

A. Nkoue Tong

Départment of Microbiology, Laboratory of Microbiology, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon.

M. R. Djoumene Kenne

Départment of Microbiology, Laboratory of Microbiology, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon.

R. Tchikoua

Départment of Microbiology, Laboratory of Microbiology, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon.

S. L. Sado Kamdem *

Départment of Microbiology, Laboratory of Microbiology, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon.

François-Xavier Etoa

Départment of Microbiology, Laboratory of Microbiology, University of Yaoundé I, P.O. Box812, Yaoundé, Cameroon.

*Author to whom correspondence should be addressed.


Aim: Starchy products are very important in the fight against food insecurity in developing countries, where they are widely consumed. This study is aimed to assess the impact of cooking and storage methods on the spoilage of some cooked starchy products by spore-forming bacteria

Place and Duration: Department of Microbiology of the University of Yaoundé I between
June 2020 and September 2022

Methodology: In order to better understand the alteration of these foods by spore-forming bacteria, a survey was carried out among some households and restaurant owners in the city of Yaoundé to determine the cooking and preservation methods for cassava, maize and rice couscous. Then, cooking parameters and conservation modalities from the survey were simulated in the laboratory in order to assess the deactivation kinetics of Bacillus cereus.

Results: It emerged from this experiment that cooking time varies according to the foodstuffs, the most important being maize. The packaging is mainly done in non-food plastic bags, and the thermos is used more often than the fridge and the pot. Cassava flour was more contaminated in aerobic spore (1.105 spores/g) than maize and rice (5.103 and 9.103 spores/g) after cooking of different starchy food enriched with Bacillus cereus spores, their inactivation was more important in rice followed by cassava and maize couscous as indicated by the Weibull scaleparameters which were respectively 1.1; 0.52 and 0.08. It was also noted that increase in cooking time also reduced the time for spores to initiate outgrowth and this time was affected by the type of flour. These two parameters also affected the time of spore outgrowth to 5logufc/g.

Conclusion:  Storage in the fridge is still the best way to store food, but the thermos flask is more often used. The use of electric thermostats set at temperatures around 60°C would increase the optimal shelf life and significantly reduce the risk of spoilage and foodborne illness.

Keywords: Starchy food, B. cereus spores, spoilage

How to Cite

Tong , A. N., Kenne , M. R. D., Tchikoua , R., Kamdem , S. L. S., & Etoa , F.-X. (2023). Effect of Food Matrix, Cooking and Preservation Modalities on the Outgrowth of Bacillus cereus Spores in Some Starchy Foods. Journal of Advances in Microbiology, 23(3), 1–12. https://doi.org/10.9734/jamb/2023/v23i3710


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FAO. Global food losses and food waste, Interpack in : Prévention du gaspillage et des pertes des produits de grande consommation : Le rôle clé de l’emballage. Conseil National Français de l’Emballage. 2011a;3. French

Pouokam GB, Saha Foudjo BU, Chi Samuel, Philomina Fankam Yamgai, Kamda Silapeux A, Joel Taguemkam Sando, Fankam Atonde G, Chiara Frazzoli. Contaminants in foods of animal origin in Cameroon: A One Health vision for Risk Management “from Farm to Fork”; 2017. DOI: 10.3389/fpubh.2017.00197.

Younoussa D, Momar TG, Mama S, Praxède GD, Amadou K, Jean-Paul B, Georges L. Importance nutritionnelle du manioc et perspectives pour l’alimentation de base au Sénégal (synthèse bibliographique). Biotechnol. Agron. Soc. Environ. 2013;17(4) :634-643. French

Krabi ER, Assamoi AA, Ehon AF. Production d’attieke (couscous à base de manioc fermente) dans la ville d’Abidjan. European Scientific Journal. 2015;(11)15:1857 – 7881. French

Thierry G, Tchuenga S, Frédéric S. Le maïs : une céréale à multiples usages au Cameroun sous la menace des contraintes climatiques et de ravageurs. Afrique SCIENCE. 2017;13(6): 177 – 188.French

Lee HY, Chai LC, Tang SY, Jinap S, Ghazali FM, Nakaguchi Y, Nishibuchi M, Son R. Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. Food Control. 2009;20: -1071.

Dolores R, Cristina M. Rosell, Antonio M. Risk of Bacillus cereus in relation to rice and derivatives. Foods. 2021;10:302. Available :https://doi.org/10.3390/foods10020302

Cadel SS, DeBuyser ML, Vignaud ML, Dao TT, Messio S, Pairaud S, Hennekinne JA, Pihier N, Brisabois A. Toxi-infections alimentaires collectives à Bacillus cereus : bilan de la caractérisation des souches de 2006 à 2010 Bulletin épidémiologique, santé animale et alimentation /Spécial Risques Alimentaires Microbiologiques. 2012;50:57-61. French

Di biase M Study on spore-forming Bacillus species involved in break spoilage, contamination risk evaluation and bio-preservation tool. Università Degli Studi Di Bari, Aldo Moro; 2013.

Carter L, Chase HR, Gieseker CM, Hasbrouck NR, Stine CB, Khan A, Ewing-Peeples LJ, Tall BD, Gopinath GR. Analysis of enterotoxigenic Bacillus cereus strains from dried foods using whole genome sequencing, multi-locus sequence analysis and toxin gene prevalence and distribution using endpoint PCR analysis. International Journal of Food Microbiology. 2018;284: 31-39.

Yu S, Yu P, Wang J, Li C, Guo H, Liu C, Kong L, Yu L, Wu S, Lei T, Chen M, Zeng H, Pang R, Zhang Y, Wei X, Zhang J, Wu Q, Ding Y. A study on prevalence and characterization of Bacillus cereus in ready-to-eat foods in China. Frontiers in Microbiology. 2020;10:3043.

Mohammed Ziane. Caractérisation, identification et étude de la thermorésistance de souches de Bacillus cereus isolées de semoule de couscous. "THESE" : Universite Aboubekr Belkaid Tlemcen; 2015.French

Etoa FX, Nkoue Tong A, Essia Ngang JJ, Sado Kamdem SL. Effect of selected conditions on spore populations outgrowth dynamics and time to single spore outgrowth distribution in Bacillus subtilis and Bacillus cereus spores population. Microbiology Research Journal International. 2017;18(2): 1-16.

Christian A, Rudi F. Effect of sporulation medium and its divalent cation content on the heat and high-pressure resistance of Clostridium botulinum type E spores. Food Microbiology. 2014; 44:156-167

ISO 7932 NF EN. Microbiologie des aliments – Méthode horizontale pour le dénombrement des Bacillus cereus présomptifs – Technique par comptage des colonies à 30°C. ISO/IEC 17025 :2005. General requirements for the competence of testing and calibration laboratories; 2005. Available:http://www.iso.org/iso/catalogue_detail.htm? csnumber=39883

(Date accessed: 15 June 2016). French

Baranyi J, Roberts T. Mathematics of predictive food microbiology. International. Journal of Food. Microbiology. 1995;26:199-218.

FAO. Pertes et gaspillages alimentaires dans le monde – Ampleur, causes et prévention. Rome; 2012. French

Ehling-Schulz M, Vukov N, Schulz A, Shaheen R, Andersson M, Martlbauer E, et al. Identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic Bacillus cereus. Applied. Environment And Microbiology. 2005;71:105–113. DOI: 10.1128/AEM.71.1.105-113. 2005

Ehling-Schulz M, Frenzel E, Gohar M. Food-bacteria interplay: Pathometabolism of emetic Bacillus cereus. Frontiers in Microbiology. 2015;6:704.

Ezo’o Mengo F. Impact de la flore sporulée bactérienne des cossettes de manioc rouies sêchées sur la durée de rouissage du manioc. Mémoire de Master. 2010;56. French

Singh J, Owen J Mc, Harinder S. Physico-chimical and morphological characteristics of New Zealand Taewa (maori potato) starches. Carbohydrate Polymer; 2006.

Parisa F, Kasiviswanathan M, Kurt A. Rosentrater Functional and Structural Properties of Corn, Potato, and Cassava Starches as Affected by a Single-Screw Extruder, International Journal of Food Properties. 2016;19(4):768-788. DOI: 10.1080/10942912.2015.1042112

Rifqi LP, Junianto. Banana leaves as a natural food packaging: A review. Global Scientific Journal. 2021;9:625-628, Online: ISSN 2320-9186

Victor R, Ron P , Amit S, Bettina K ,Haya F. Microperforated compostable packaging extends shelf life of ethylene-treated banana fruit. Foods. 2022;1086:1-11. DOI:https://doi.org/ 10.3390/foods11081086

FDA (Food and Drug Administration) – bacteriological analytical manual – Chapter 14 : Bacillus cereus.

Byrne B, Dunne G, Bolton DJ. Thermal inactivation of Bacillus cereus and Clostridium perfringens vegetative cells and spores in pork luncheon roll. Food Microbiology; 2006.

Sarang S, Sastry SK, Knipe L. Electrical conductivity of fruits and meats during ohmic heating. J Food Eng. 2008;87:351–356.

Guillermo C ID, Santiago C, Pilar M. Physiology of the inactivation of vegetative bacteria by thermal treatments: Mode of action, Influence of Environmental Factors and Inactivation Kinetics. Foods. 2017;6:107. DOI:10.3390/foods6120107

Chmal-Fudali E, Papiewska A. The possibility of thermal inactivation of Alicyclobacillus acidoterrestris spores in fruit and vegetable juices. Biotechnology Food Sciences. 2011;75:87-96

Samapundo S, Heyndrickx M, Xhaferi R, De Baenst I. and Devlieghere F. The combined effect of pasteurization intensity, water activity, pH and incubation temperature on the survival and outgrowth of spores of Bacillus cereus and Bacillus pumilus in artificial media and food products. International Journal of Food Microbiology. 2014;181:10-18.

Pandey R, Ter Beek A, Vischer NOE, Smelt JPPM, Brul S, Manders EMM. Live cell imaging of germination and outgrowth of individual Bacillus subtilis spores; the effect of heat stress quantitatively analyzed with SporeTracker. PLOS ONE. 2013;8(3):58-72.

Alicja K, Xiao Y, Boekhorst J, WellsBennik M, Nierop M, Abee T. Analysis of germination capacity and germinant receptor (sub) clusters of genome sequenced Bacillus cereus environmental isolates and model strains. Applied Environment and Microbiology. 2017;83:1- 16.

Valero M, Hernandez-Herrero LA, Giner MJ. survival isolation and characterisation of a psychrotrophic Bacillus cereus strain from a mayonnaise-based ready to eat vegetable salad. Food Microbiology. 2007;24: 671-677.

Rym Nouria Benamara. Identification et caractérisation de spores de Bacillus cereus isolées de fromages fondus fabriqués en Algérie ; 2017. French

Kombou MN, Joseph A. Composition protéique et minérale de quelques plats traditionnels cuisinés en milieu urbain (Yaoundé). Science and Technoldgy Review, 1984;1: 31-44.

Anse-ciqual. Table de composition des aliments : 2020 ;French ;.

Guinebretière S, Auger N, Galleron M, Contzen B, De Sarrau M-L, Granum D, Lereclus P, De Vos, Nguyen-The C, Sorokin A. Bacillus cytotoxicus sp.nov.is a novel thermotolerant species of the Bacillus cereus Group occasionally. International Journal System Evolution Microbiology. 2013;63:31-40.

European Food Safety Authority (EFSA). Risks for public health related to the presence of Bacillus cereus and other Bacillus spp. including Bacillus thuringiens is in foodstuffs. EFSA J. 2016;14:4524.

Bursova S, Necidova L, Harustiakova D. Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula. Food Control. 2018;93:334-343.

Rouzeau-Szynalski K, Stollewerk K, Messelhausser U, Ehling-Schulz M. Why be serious about emetic Bacillus cereus: Cereulide production and industrial challenges. Food Microbiology. 2020;85.

Glasset B, Herbin S, Guillier L, Cadel-Six S, Vignaud ML, Grout J, Pairaud S, Michel V, Hennekinne JA, Ramarao N, Brisabois A. Bacillus cereus-induced food-borne outbreaks in France, 2007 to 2014: epidemiology and genetic characterisation. Eurosurveillance. 2016; 21(48):30413. DOI:http://dx.doi.org/10.2807/1560-7917. ES.2016.21.48.30413. PMid:27934583.