In-vitro and In-vivo Anti-Candidal Effect of Cnidoscolus aconitifolius Leaves Extracts

J. C. Adindu *

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

C. O. Anyamene

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

C. U. Ezebialu

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

D. C. Chukwukaelo

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

C. F. Chude

Department of Zoology, Nnamdi Azikiwe University, Nigeria.

L. E. Eruala

Department of Epidemiology, Biostatistics and Occupational Health, McGill University, Canada.

A. Umeofor

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Candidiasis is an opportunistic infection caused by pathogens of the genus Candida. Due to the increase in antifungal resistance by Candida species, there is a need for alternatives in its treatment. Cnidoscolus aconitifolius is a medicinal plant with vast nutritional and antimicrobial properties. This study evaluated the in-vitro and in-vivo anti-candidal effect of Cnidoscolus aconitifolius (spinach tree) leaves extract. Clinical isolates of Candida albicans, Candida krusei, Candida tropicalis, Candida dublinensis, Candida glabrata, and Candida parapsilosis were used in this study and Cold maceration technique was employed in the plant extraction process, to obtain its ethanolic, methanolic, and aqueous extract. The agar well diffusion method and 2-fold dilution process -to obtain 250 mg/ml to 15.625mg/ml of the extracts -were used for the antimicrobial sensitivity test and three weeks old female Wistar rats infected with 20µl of standardized C. albicans were used for the in-vivo evaluation. Statistical analysis was done using Statistical package for Social Sciences (SPSS) version 22. In-vitro assay revealed ethanolic extract as the most potent extract with the highest inhibition zone diameter of 12.67±1.15c, 11.67±1.15c, and 12.33±0.58c at 250mg/ml against C. tropicalis, C. krusei2, and C. parapsilosis respectively. C. parapsilosis was the most sensitive to all the extracts. In-vivo, the disappearance of disease symptoms and progressive decrease in rats Candida burden- from 6.35×103 CFU/ml obtained after infection to 2.15×103CFU/ml after treatment revealed the ability of the plant to treat candidiasis. The study suggests that extract of Cnidoscolus aconitifolius leaves could act individually or synergistically as effective drug candidates in the treatment of candidiasis; thus if its individual antifungal constituents are purified, this plant could be the next major anti-candidal agent.

Keywords: Candidiasis, Cnidoscolus aconitifolius, female wistar rats, candida species, anti-candidal effect


How to Cite

Adindu, J. C., C. O. Anyamene, C. U. Ezebialu, D. C. Chukwukaelo, C. F. Chude, L. E. Eruala, and A. Umeofor. 2023. “In-Vitro and In-Vivo Anti-Candidal Effect of Cnidoscolus Aconitifolius Leaves Extracts”. Journal of Advances in Microbiology 23 (6):27-37. https://doi.org/10.9734/jamb/2023/v23i6729.

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References

Ezeadila JO, Okoli I, Oyeka CA. Antifungal resistance pattern of Candida species isolated from high vaginal swabs of women attending a hospital in Enugu State, Nigeria. Journal of Advances in Microbiology. 2020;20(9):62-72.

Yang YL. Virulence factors of Candida species. Journal of Microbiology, Immunology and Infection. 2003;36(4):223-228.

Raesi, Vanani A, Madavinia M, Kalantari H, Khoshnood S, Shirani M. Antifungal effect of the effect of Securigeera securidaca (L.) vaginal gel on Candida species. Current Medical Mycology. 2019;5(3):31-35.

Sexton JA, Brown V, Johnston M. Regulation of sugar transport and metabolism by the Candida albicans Rgt1 transcriptional repressor. Yeast. 2007;24 (10):847–860.

Fang J, Huang B, Ding Z. Efficacy of antifungal drugs in the treatment of oral candidiasis: A Bayesian network meta-analysis. The Journal of Prosthetic Dentistry. 2021;125(2):257-265.

Pfaller, Michael A, Diekema DJ, Turnidge JD, Castanheira M, Jones RN. Twenty years of the sentry antifungal surveillance program: Results for Candida species from 1997--2016. Open Forum Infectious Diseases. 2019;6(1):S79--S94.

World Health Organization (WHO). WHO monographs on selected medicinal plants. 2005;4.

Fagbohun ED, Egbebi AO, Lawal OU. Phytochemical screening, proximate analysis and in-vitro antimicrobial activities of methanolic extract of Cnidoscolus aconitifolius leaves. International Journal of Pharmaceutical Sciences Review and Research. 2012;13:1.

Rajore S, Batra A. Jatropha curcas L: A plant of immense potential value. The Journal of Economic and Taxonomic Botany. 2003;27:36-41.

Iwalewa EO, Adewunmi CO, Omisore NO, Adebanji OA, Azike CK. Pro-oxidant effects and cytoprotective potentials of nine edible vegetables in Southwest Nigeria. Journal of Medicinal Foods. 2005;8:539-544.

Morton JF. Atlas of medicinal plants of Middle America: Bahamas to Yucatan. C.C. Thomas, Springfield, IL. 1981;2:1448.

Viswanathan MBG, Ananthi JDJ, Venkatesan N. Review on Jatropha. International Journal of Research and Innovation in Social Science (IJRISS). 2018;2:8.

Bautista-Robles V, Guerrero-Reyes G, Sánchez-Torres, G.I., de Jesús Parada-Luna, F., Barrios-Gutiérrez, J.J., VázquezCerero, D., Martínez-Sala G, Siliceo-Murrieta JI, González-Villoria RAM, Keita H. Cnidoscolus aconitifolius: Therapeutic use and phytochemical properties. Literature review. Rev. Fac. Med. 2020;68(3):446-452.

Jayashree KV, Gopukumar ST. Antimicrobial potential of various extracts of Cnidoscolus aconitifolius latex. Journal of Applied Science and Computations. 2018;5:12.

Hamid AA, Oguntoye SO, Negi AS, Ajao A, Owolabi NO. Chemical constituents, antibacterial, antifungal and antioxidant activities of the aerial parts of Cnidoscolus aconitifolius. Ife Journal of Science. 2016;18:2.

Senjobi CT, Moody JO, Ettu AO. Antimicrobial and Cytotoxic Effects of Cnidoscolus aconitifolius (miller) Johnson. Journal of Agriculture and Biological Sciences. 2011;2(2):021-025.

Oyegbemi AA, Odetola AA. Hepato protective effects of ethanolic extract of Cnidoscolus aconitifolius on paracetamol-induced hepatic damage in rats. Pakistan Journal of Biological Science. 2010;13: 164-169.

Buss AD, Butler MS. (Eds.). Natural product chemistry for drug discovery. The Royal Society of Chemistry, Cambridge. 2010:153.

Perez C, Pauli M, Bazerque P. Antibiotic assay by agar-well diffusion method. Acta Biologiae Et Medicinae Experimentalis. 1990;15:113-115.

Cheesbrough M. District laboratory practice in tropical countries, Part 2, (2nd ed.), Cambridge University Press, Cambridge, UK. 2010:137 - 138.

Marcia A Carrara, Lucelia Donatti, Edilson Damke, Terezinha IE, Svidizinski Marcia Consolaro EI, Marcia R Batista. A new model of vaginal infection by Candida albicans in rats. Mycopathologia. 2010; 170:331-338.

Ikele MO, Ilikannu S, Obasi CJ, Okeke BC, Ikele BC, Nwakoby NE, Ukoha CC, Victor-Aduloju AT, Aduloju T. Antifungal activities of Alcaligenes Faecalis palm wine isolate in mice model vaginal candidiasis. International Journal of Innovative Research and Development. 2019;8(10): 112-118.

DOI:10.24940/ijird/2019/v8/i10/148261-364789-1-SM

Esther Segal, Michael Frenkel. Experimental in vivo models of candidiasis. Journal of Fungi. 2018;4:1.

Adaramoye OA, Aluko A, Oyagbemi AA. Cnidoscolus aconitifolius leaf extract protects against hepatic damage induced by chronic ethanol administration in wistar rats. Alcohol Alcohol. 2011;46(4): 451-458.

Oyagbemi AA, Ogunleye AO, Lawal TO, Azeez IO. The effect of Cnidoscolus aconitifolius on multi-drug resistant micro-organisms. African Journal of Biotechnology. 2011;10(3):413-415.

Trease EG, Evans CW. Laboratory methods in antimicrobial chemotherapy. Churchill Livingstone, Edinburgh. 1978:35-38.

Nweze EI, Eze EE. Justification for the use of Ocimum gratissimum L in herbal medicine and its interaction with disc antibiotics. BMC Complementary and Alternative Medicine. 2009;9:37.

Chipinga JV, Kamanula JF, Moyo PBB. Efficacy of Pterocarpus angolensis crude extracts against Candida krusei, Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli. Malawi Medical Journal. 2018;30(4):219-224.

Otitolaiye CA, Asokan C. GC-MS Analysis of Cnidoscolus aconitifolius leaf aqueous extracts. International Journal of Science and Research (IJSR). 2015;5:8.

Rahman MS, Anwar MN. Antibacterial and cytotoxic activity of longiverbenone isolated from the rhizome of Cyperus scarious. Bangladesh Journal of Microbiology. 2008;25:82-84.

Thangjam NM, Taijong J, Kumar A. Phytochemical and pharmacological activities of methanol extract of Artemisia vulgaris L. leaves. Clinical Phytoscience. 2020;6:1–8.

DOI: 10.1186/s40816-020-00214-8.

Alexander-lindo RL, Barrington R, Porter R, Nwokocha CR, Lattibeaudiere KG. The phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back). Clinical Phytoscience. 2020;6:1–9.

DOI: 10.1186/s40816-020-00220-w