Journal of Advances in Microbiology

Characterization and Antibiogram of Bacteria Isolated from Surgical Wounds of Patients Attending Ibrahim Badamasi Babangida Specialist Hospital Minna, Niger State, Nigeria

Surgical wounds are one of the major sources through which bacteria pathogens gain access into internal tissues. The study is therefore aimed at determining the proximate bacteria associated with infected surgical wounds of patients attending Ibrahim Badamasi Babagida specialist hospital Minna, Niger state, North central, Nigeria. Fifty samples were collected from the patient using sterile swab stick, inoculated on the prepared nutrient agar, incubated for 24 hours, morphologically observed, biochemically characterized and investigated for antibiotic sensitivity. The result for the proximate analysis indicated that thirty samples were contaminated with bacteria. Out of the 30 positive samples, three Gram negative bacteria which include Pseudomonas aeruginosa, Escherichia coli and Klebsiella ozaenae, and four Gram positive bacteria which include Staphylococcus aureus, Streptococcus pyogenes, Streptococcus agalactiae and Clostridium perfringens were isolated. The result revealed that Staphylococcus aureus has the highest prevalence, isolated from 14 samples (46.67%) followed by Pseudomonas aeruginosa, isolated from 6 samples (20%), both Streptococcus agalactiae and Streptococcus pyogenes were isolated from 3 (10%) different samples each, Escherichia coli was isolated only from 2 samples (6.67%), whereas Clostridium perfringens and Klebsiella ozaenae were isolated from just a sample (3.33%) each. From the susceptibility analysis, Klebsiella ozaenae showed the highest percentage of susceptibility and resistance to the antibiotic disk among the Gram negative while Clostridium perfringens showed the highest percentage of the sensitivity to the antibiotic disc out of the Gram positive bacteria isolates.