Isolation and Susceptibility of Microorganisms Responsible for Asymptomatic Antenatal Bacteriuria

Kousar Parveen *

Department of Pathology, Peoples University of Medical and Health Sciences (PUMHS-W), Nawabshah, Pakistan.

Sikander Ali Sial

Department of Pathology, Peoples University of Medical and Health Sciences (PUMHS-W), Nawabshah, Pakistan.

Sandhia Kumari

Department of Pathology, Peoples University of Medical and Health Sciences (PUMHS-W), Nawabshah, Pakistan.

Arooj Ali

Department of Pathology, Peoples University of Medical and Health Sciences (PUMHS-W), Nawabshah, Pakistan.

Irum Akound

Department of Pathology, Peoples University of Medical and Health Sciences (PUMHS-W), Nawabshah, Pakistan.

Amir Ali Akhound

Department of Pathology, Peoples University of Medical and Health Sciences (PUMHS-W), Nawabshah, Pakistan.

*Author to whom correspondence should be addressed.


Aim & Objective: Determination of prevalence, bacterial profile and antibiotic sensitivity of microorganisms responsible for asymptomatic UTI during pregnancy.

Methodology: The type of study is cross-sectional, Prospective type, was conducted at the PUMHS for Women Nawabshah (SBA) after approval from the Ethical Review Committee and the duration was 2 years from 1st Jan 2021-31st Dec 2022. Samples were collected by non-probability- convenient method from all pregnant women without any clinical complaints attending the Outpatient Department of Gynecology and Obstetrics PUMHS Hospital Nawabshah. The midstream urine collected by clean catch method in sterile containers was processed for DR of urine including physical, chemical, and microscopic examination. After the urine detailed report, the samples were sent for, culture by using the following media, blood, Macconkey’s, and CLED agar. Growth-positive samples were proceeding for gram staining, biochemical tests, and antibiotic sensitivity reports according to world standard methods for confirmation.

Results: Out of 477 patients 96 (20%) were positive for ASB, with the mean age of the patient being 29.32±5.74 The most common microorganisms found in the study were E.coli 67 (69.7.%), followed by Klebisella in 4(4%), proteus 9(9%), pseudomonas 8(8.3%), staphylococcus aureus 2(2%), staphylococcus saprophyticus in 4(4%), streptococcus pyogen 2(2%).

There was 100% sensitivity found for all isolated microorganisms following culture examination to following antibiotics: Ciprofloxacin, Cefuroxime, Imepenem, Ceftriaxone Amikacin, Meropenem, and Piperacillin-tazobactam; however, 52% and 45% sensitivity of E.coli and 100% sensitivity of streptococci were present for amoxicillin-clavulanic acid and ampicillin respectively.

Conclusion: A rising number of silent antenatal bacteriuria was observed in younger age women with severe maternal and fetal complications in advanced stages of pregnancy and thereafter. It is therefore suggested to routinely perform urine cultures for every suspected pregnant lady during advanced antenatal visits to diagnose and properly treat the asymptomatic bacteriuria in terms of avoiding antibiotic resistance and materno-fetal complications in the near future.

Keywords: ASB, antibiotic susceptibility, asymptomatic bacteriuria, urinary tract infection, antenatal, Isolation

How to Cite

Parveen , Kousar, Sikander Ali Sial, Sandhia Kumari, Arooj Ali, Irum Akound, and Amir Ali Akhound. 2023. “Isolation and Susceptibility of Microorganisms Responsible for Asymptomatic Antenatal Bacteriuria”. Journal of Advances in Microbiology 23 (11):23-29.


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Sial SA, Detho AB, Memon FR, Parveen K, Memon S, Bukhari S, Ahmer A. Risk Factors during Pregnancy for Asymptomatic Bacteriuria. Journal of Pharmaceutical Research International. 2021 May 8;33(28B):21-6.

Sheppard M, Ibiebele I, Nippita T, Morris J. Asymptomatic bacteriuria in pregnancy. Australian and New Zealand Journal of Obstetrics and Gynaecology; 2023 May 8.

Nteziyaremye J, Iramiot SJ, Nekaka R, Musaba MW, Wandabwa J, Kisegerwa E, Kiondo P. Asymptomatic bacteriuria among pregnant women attending antenatal care at Mbale Hospital, Eastern Uganda. PloS One. 2020 Mar 19;15(3): e0230523.

Wingert A, Pillay J, Sebastianski M, Gates M, Featherstone R, Shave K, et al. Asymptomatic bacteriuria in pregnancy: Systematic reviews of screening and treatment effectiveness and patient preferences. BMJ Open. 2019 Mar 1;9(3): e021347.

Werter DE, Kazemier BM, van Leeuwen E, de Rotte MC, Kuil SD, Pajkrt E, Schneeberger C. Diagnostic work-up of urinary tract infections in pregnancy: study protocol of a prospective cohort study. BMJ Open. 2022 Sep 1;12(9):e063813.

Hafeez S, Hafeez Z, Hafeez S, Riaz S, Yousuf A. Frequency of Asymptomatic Bacteriuria and its Causative Organisms among Pregnant Women Visiting for Antenatal Checkup in Outpatient Department of Sims/Services Hospital. Age (Years). 26:4-08.

Dube R, Al-Zuheiri ST, Syed M, Harilal L, Zuhaira DA, Kar SS. Prevalence, Clinico-Bacteriological Profile, and Antibiotic Resistance of Symptomatic Urinary Tract Infections in Pregnant Women. Antibiotics. 2022 Dec 25;12(1):33.

Taha LH, Almahmood AA, Farhan RK. Distribution of asymptomatic bacteriuria among women using Intrauterine Devices (IUDs) according sociodemographic characteristics factors in Takrit, Iraq. Iraq Medical Journal. 2021 Dec 26;5(4).

Edae, M., Teklemariam, Z., Weldegebreal, F. and Abate, D., 2020. Asymptomatic bacteriuria among pregnant women attending antenatal care at Hiwot Fana specialized university hospital, Harar, eastern Ethiopia: magnitude, associated factors, and antimicrobial susceptibility pattern. International Journal of Microbiology; 2020.

Ned CB, Petitti M, DeWitt TG, Russell H. Screening for asymptomatic bacteriuria in adults: U.S. Preventive services task force reaffirmation recommendation statement, Annals of Internal Medicine. 2008; 149(4).

Mwei MK, Mchome B, John B, Maro E. Asymptomatic bacteriuria among pregnant women attending antenatal clinic at Kilimanjaro Christian Medical Centre in Northern Tanzania. Clin Pract. 2018;15(6): 917-22.

Kafi AA, Khatun M, Shoshi BN. The Association of Asymptomatic Urinary Tract Infection (UTI) with Diabetes Mellitus (DM) in Pregnant Women.

Sujatha R, Nawani M. Prevalence of asymptomatic bacteriuria and its antibacterial susceptibility pattern among pregnant women attending the antenatal clinic at Kanpur, India. Journal of clinical and diagnostic research: JCDR. 2014 Apr;8(4):DC01.

Ali R, Afzal U, Kausar S. Asymptomatic bacteriuria among pregnant women. Annals of Punjab Medical College (APMC). 2011 Dec 12;5(2):155-8.

Sonkar N, Banerjee M, Gupta S, Ahmad A. Asymptomatic bacteriuria among pregnant women attending tertiary care hospital in Lucknow, India. Dubai Medical Journal. 2021 Mar 11;4(1):18-25

Celen S, Oruç AS, Karayalçin R, Saygan S, Unlü S, Polat B, Danişman N. Asymptomatic bacteriuria and antibacterial susceptibility patterns in an obstetric population. ISRN Obstet Gynecol. 2011; 2011:721872.

DOI: 10.5402/2011/721872

Epub 2011 Jan 24.

Akerele J, Abhulimen P, Okonofua F. Prevalence of asymptomatic bacteriuria among pregnant women in Benin City, Nigeria. J Obstet Gynaecol. 2001 Mar; 21(2):141-4. DOI: 10.1080/0144361002002603 PMID: 12521882.

Karikari AB, Saba CKS, Yamik DY. Assessment of asymptomatic bacteriuria and sterile pyuria among antenatal attendants in hospitals in northern Ghana. BMC Pregnancy Childbirth. 2020;20: 239. Available:

Nicolle LE. Screening for asymptomatic bacteriuria in pregnancy. Ottawa Health, Canada: Canadian Guide on Preventive Health Care. 1994;100–106.

Elzayat MA, Barnett-Vanes A, Dabour MF, Cheng F. Prevalence of undiagnosed asymptomatic bacteriuria and associated risk factors during pregnancy: a cross-sectional study at two tertiary centers in Cairo, Egypt. BMJ open. 2017 Mar 1;7(3):e013198.

Ullah MA, Barman A, Siddique MA, Haque AK. Prevalence of asymptomatic bacteriuria and its consequences in pregnancy in a rural community of Bangladesh. Bangladesh Medical Research Council Bulletin. 2007;33(2):60-4.

Ali IE, Gebrecherkos T, Gizachew M, Menberu MA. Asymptomatic bacteriuria and antimicrobial susceptibility pattern of the isolates among pregnant women attending Dessie referral hospital, Northeast Ethiopia: A hospital-based cross-sectional study. Turkish Journal of Urology. 2018;44(3):251.

Jayalakshmi J, Jayaram VS. Evaluation of various screening tests to detect asymptomatic bacteriuria in pregnant women. Indian J Pathol Microbiol. 2008 Jul-Sep;51(3):379-81. DOI: 10.4103/0377-4929.42516 PMID: 18723963.

Jain V, Das V, Agarwal A, Pandey A. Asymptomatic bacteriuria & obstetric outcome following treatment in early versus late pregnancy in north Indian women. Indian J Med Res. 2013 Apr; 137(4):753-8. PMID: 23703344; PMCID: PMC3724257.

Patnaik M, Panigrahi K, Das B, Pathi B, Poddar N, Lenka PR, Pattnaik D. Prevalence, risk factors and causative organisms of asymptomatic bacteriuria in pregnancy. Int J Adv Med. 2017 Sep; 4(5):1348-54.

Vicar EK, Acquah SE, Wallana W, Kuugbee ED, Osbutey EK, Aidoo A, Acheampong E, Mensah GI. Urinary Tract Infection and Associated Factors among Pregnant Women Receiving Antenatal Care at a Primary Health Care Facility in the Northern Region of Ghana. International Journal of Microbiology; 2023.

Tadesse E, Teshome M, Merid Y, Kibret B, Shimelis T. Asymptomatic urinary tract infection among pregnant women attending the antenatal clinic of Hawassa Referral Hospital, Southern Ethiopia. BMC Res Notes. 2014 Mar 17;7:155. DOI: 10.1186/1756-0500-7-155