Internal Transcribed Spacers (ITS) Based Identification of Trichoderma Isolates and Biocontrol Activity against Fusarium oxysporum and Chitinase Activity
Jaygendra Kumar *
Department of Agricultural Biotechnology, College of Agriculture, SVP University of Ag. and Tech, Modipuram, Meerut-250110, Uttar Pradesh, India.
Mukesh Kumar
Department of Agricultural Biotechnology, College of Agriculture, SVP University of Ag. and Tech, Modipuram, Meerut-250110, Uttar Pradesh, India.
Vaishali
Department of Agricultural Biotechnology, College of Agriculture, SVP University of Ag. and Tech, Modipuram, Meerut-250110, Uttar Pradesh, India.
Akash Tomar
Department of Recombination Techniques, College of Biotechnology, SVP University of Ag. and Tech., Modipuram, Meerut-250110, Uttar Pradesh, India.
Puspendra Kumar
Department of Agricultural Biotechnology, College of Agriculture, SVP University of Ag. and Tech, Modipuram, Meerut-250110, Uttar Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Twelve Trichoderma spp. isolated from different locations in Uttar Pradesh, India. Internal transcribed spacer (ITS) amplification and sequencing were used to identify isolates of Trichoderma that showed 99–100% identification with Trichoderma harzianum, Trichoderma asperellum, and Trichoderma longibrachiatum, the three species of Trichoderma. Tests were conducted in Vitro to evaluate the biocontrol potential of Trichoderma isolates against Fusarium species. After seven days of incubation, the isolate TBT6 (T. harzianum) exhibited the greatest antagonism against Fusarium oxysporum, with inhibition rates of 87.1%) by TBT7 (82.2%), and the least amount of inhibition (59.7%) by T. longibrachiatum isolate TBT10. Under field conditions, the antagonistic T. harzianum isolate TBT6 can be employed as a bio-control agent against Fusarium oxysporum. and for the development of formulations based on Trichoderma. Using the dual plate approach, twelve highly antagonistic Trichoderma isolates were selected for chitinolytic activity. Observing the breakdown of chitin substrates, Trichoderma isolates TBT6 was shown to have strong chitinolytic enzyme activity, making it a good candidate for endochitinase gene isolation. Using two particular primers, the genomic DNA of the Trichoderma isolate TBT6 was isolated and amplified.
Keywords: Trichoderma, internal transcribed spacers (ITS), antagonism, Fusarium oxysporum, chitinase activity, biocontrol