Isolation, Morphological Characterization and Screening of Yeast Isolates from Different Fruit Samples of Raichur District, Karnataka, India
Shruthi C *
Department of Agricultural Microbiology, University of Agricultural Sciences, Raichur-584104, Karnataka, India.
Yallappa M
Department of Agricultural Microbiology, University of Agricultural Sciences, Raichur-584104, Karnataka, India.
Nagraj M Naik
Department of Agricultural Microbiology, University of Agricultural Sciences, Raichur-584104, Karnataka, India.
Pampangouda
Department of Agricultural Microbiology, University of Agricultural Sciences, Raichur-584104, Karnataka, India.
Pampanna Y
Department of Horticulture, College of Agriculture, University of Agricultural Sciences, Raichur, Karnataka - 584104, India.
*Author to whom correspondence should be addressed.
Abstract
A laboratory experiment was conducted in the Department of Agricultural Microbiology, College of Agriculture Raichur and Bheemarayangudi for isolation, characterization and screening of yeast isolates from different fruit samples of Raichur district. Fruit samples like Fig, Papaya, Pomegranate, Dragon fruit and Grapes were collected from different places of Raichur district. In total 26 yeast isolates were isolated from collected fruit samples all yeast isolates showed colour characteristics like dull white, white milky, creamish-white, pink and orange. The population of yeast isolates ranged from 2.00 to 58.50 cfu/ml of fruit samples. All 26 yeast isolates were positive for sugar fermentation test, different carbon utilization test, ethanol, glucose and pH tolerance test. The yeast isolate Raichur Papaya Yeast- 5 shown better results (2.510@10% v/v, 3.055 @30% and 2.862@ pH 6 respectively).
Keywords: Yeast, RPAY- 5, sugar fermentation, ethanol tolerance, glucose tolerance, PH tolerance