Isolation, Identification, and Characterization of Plant Growth-Promoting Rhizobacteria from Sugarcane (Saccharum Officinarum) Rhizosphere
Divyesh Vasava *
Department of Microbiology, Arihant Bio Science (India) Pvt. Ltd., Plot No. 5145, Nr. Prime industries, Paras chokdi, GIDC Estate, Ankleshwar, Gujarat-393002, India.
Aditi Pandya
Department of Microbiology, Arihant Bio Science (India) Pvt. Ltd., Plot No. 5145, Nr. Prime industries, Paras chokdi, GIDC Estate, Ankleshwar, Gujarat-393002, India.
Sapna Tiwari
Department of Microbiology, Arihant Bio Science (India) Pvt. Ltd., Plot No. 5145, Nr. Prime industries, Paras chokdi, GIDC Estate, Ankleshwar, Gujarat-393002, India.
Laxmina Mishra
Department of Microbiology, Arihant Bio Science (India) Pvt. Ltd., Plot No. 5145, Nr. Prime industries, Paras chokdi, GIDC Estate, Ankleshwar, Gujarat-393002, India.
Bhavesh Patel
Arihant Bio Science (India) Pvt. Ltd., Plot No. 5145, Nr. Prime industries, Paras chokdi, GIDC Estate, Ankleshwar, Gujarat-393002, India.
Narendra Patel
Arihant Bio Science (India) Pvt. Ltd., Plot No. 5145, Nr. Prime industries, Paras chokdi, GIDC Estate, Ankleshwar, Gujarat-393002, India.
*Author to whom correspondence should be addressed.
Abstract
The use of biofertilizers and biopesticides is the foundation of modern sustainable agricultural techniques. The rhizosphere of sugarcane (Saccharum officinarum) may provide rhizobacteria the ability to fertilize and repel pests. This research focused at the plant growth promotion (PGP) abilities of bacteria found in the rhizosphere of sugarcane. For example, how they solubilize phosphate, produce IAA, produce nitrogen, and help seeds sprout. Soil samples were taken from different types of sugarcane rhizosphere in the Ankleshwar area to obtain isolates. For further study and molecular identification using the 16S rRNA gene sequence, one strain of bacteria was picked because it could solubilize phosphate and produce IAA. This confirms the isolate's identity as Klebsiella pneumoniae. The isolate had a phosphate solubilization index of 2.11, and the PKVK broth gave off 91.34±2 μg/ml of phosphate, which was calculated. After 72 hours of incubation at room temperature, 61.54±2 μg/ml of IAA without tryptophan was generated. The test on seed germination showed that treating plants with Glycine max, Solanum melongena, Solanum lycopersicum, Capsicum annuum, and Oryza sativa bacteria increased plant height, dry weight, and fresh weight more than the control group. Solanum lycopersicum exhibited the highest rate of germination (100%). The infected agricultural seeds' better seedling characteristics suggested that this isolate may be used to a biofertilizer formulation for environmentally friendly production.
Keywords: PGPR, Klebsiella pneumoniae, N2 fixation, IAA, phosphate solubilisation