Antibiotic Susceptibility and Molecular Characterization of Pseudomonas fluorescens from Hospital Biomedical Waste in Kalahandi, India
Ghanashyam Behera
Department of Botany, Maa Manikeshwari University, Bhawanipatna, 766001, Odisha, India.
Nihar ranjan nayak
Department of Botany, Maa Manikeshwari University, Bhawanipatna, 766001, Odisha, India.
Ambika sahu
Department of Botany, Maa Manikeshwari University, Bhawanipatna, 766001, Odisha, India.
Roshan Dash
Department of Botany, Maa Manikeshwari University, Bhawanipatna, 766001, Odisha, India.
Soumya Ranjan Sahu
Department of Botany, Maa Manikeshwari University, Bhawanipatna, 766001, Odisha, India.
Sujata Meher
Department of Botany, Maa Manikeshwari University, Bhawanipatna, 766001, Odisha, India.
Priti mayee Khura
Department of Botany, Maa Manikeshwari University, Bhawanipatna, 766001, Odisha, India.
Priyanka Bhoi
Department of Botany, Maa Manikeshwari University, Bhawanipatna, 766001, Odisha, India.
Subrat Kumar Panigrahi *
Department of Zoology, Maa Manikeshwari University, Bhawanipatna, 766001, Odisha, India.
*Author to whom correspondence should be addressed.
Abstract
The prevalent application of antibiotics and the rise of antibiotic resistance in healthcare settings present challenges. However, antibiotics and antibiotic resistance as potential environmental threats and hazards have received less attention. The increasing prevalence of antibiotic resistance among various organisms is a significant concern for modern medicine. Variations in prevalence and resistance rates highlight the need for standardized monitoring and control strategies. Our findings, based on 16S rRNA phylogenetic analysis, provide valuable insights into bacterial classification and evolutionary relationships. Additionally, Pseudomonas fluorescens is emerging as a multidrug-resistant (MDR) pathogen, with resistance patterns influenced by geographical factors and antibiotic exposure. The complete resistance of the MDR-08 strain to five antibiotics is particularly concerning, underscoring the urgent need for stricter antibiotic stewardship and improved hospital waste management practices.
Keywords: Pseudomonas fluorescens, antibiotic Susceptibility, biomedical waste, Kalahandi, antibiotic resistance