Proposed Use of Limocide 60 ME (An Extract of Sweet Orange Essential Oil) to Control Pathogens of Crown Rot in Dessert Bananas
KOUAME Konan Didier
Plant Physiology and Pathology Pedagogy and Research Unit of the Biotechnology, Agriculture and Biological Resources Development Laboratory, Biosciences UFR, Université Félix Houphouët-Boigny, Côte d'Ivoire.
N’GUESSAN Patrick Henri
Plant Physiology and Pathology Pedagogy and Research Unit of the Biotechnology, Agriculture and Biological Resources Development Laboratory, Biosciences UFR, Université Félix Houphouët-Boigny, Côte d'Ivoire.
KASSI Koffi Jean Fernand Martial
Plant Physiology and Pathology Pedagogy and Research Unit of the Biotechnology, Agriculture and Biological Resources Development Laboratory, Biosciences UFR, Université Félix Houphouët-Boigny, Côte d'Ivoire.
YAO Kouadio Jacques Edouard *
Agricultural Production Improvement Laboratory, Faculty of Agroforestry, Jean Lorougnon Guédé University, Daloa, Côte d'Ivoire.
KOUAME Koffi Gaston
Department of Plant Biology, Université Péléforo Gon COULIBALY(UPCG), Korhogo, Côte d'Ivoire.
SYLLA Tata
Plant Physiology and Pathology Pedagogy and Research Unit of the Biotechnology, Agriculture and Biological Resources Development Laboratory, Biosciences UFR, Université Félix Houphouët-Boigny, Côte d'Ivoire.
KONE Klinninga Noël
Department of Plant Biology, Université Péléforo Gon COULIBALY(UPCG), Korhogo, Côte d'Ivoire.
KANGA N’guessan Martial
Agronomic, Forestry and Environmental Engineering Training and Research Unit at the University of Man, Côte d'Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Background: In Côte d'Ivoire, dessert banana production has been estimated at around 450,000 tonnes in 2019, representing 7% of agricultural Gross Domestic Product (GDP) and 3% of national GDP. However, it has been repeatedly observed that the application of synthetic fungicides for pest control would have numerous harmful consequences for man and his environment. Objective: The main aim of this study is to demonstrate the efficacy of Limocide “a biological fungicide” against crown rot of dessert banana. Methodology: The biological product used for the treatments was Limocide 60 ME, an extract of essential oil from sweet orange. Pure colonies aged 6 to 14 days were identified using the Botton et al. determination key (1990). Limocide 60 ME was only tested at 500 ppm because of its effectiveness. As for PRBB, it was incorporated to obtain concentrations of 250, 500, 750, 1500 and 2500 ppm and 100, 150, 250, 400. These products were compared with citric acid. The effect of the different products was determined from the calculated rate of mycelial growth inhibition. The data collected were analyzed using Statistica version 7.1 software. Results: Three fungal strains were identified: Fusarium oxysporium, Fusarium solani and colletotrichum musae. These were identified as responsible for crown rot disease. Control tests carried out in the laboratory also demonstrated that Limocide 60 ME at 500 ppm and PRBB at 400 ppm have very significant antifungal activity for disease control.
Keywords: Côte d'Ivoire, dessert banana, Limocide 60 ME, crown rot