Journal of Advances in Microbiology <p style="text-align: justify;"><strong>Journal &nbsp;of Advances in Microbiology&nbsp;(ISSN:&nbsp;2456-7116)&nbsp;</strong>aims to publish&nbsp;high quality papers (<a href="/index.php/JAMB/general-guideline-for-authors">Click here for Types of paper</a>)&nbsp;in all areas of Microbiology. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.&nbsp;</p> SCIENCEDOMAIN international en-US Journal of Advances in Microbiology 2456-7116 Hygienic Conditions and Quality of Dishes Cooked and Sold by Women Traders in the Town of Korhogo <p>The aim of this study is to evaluate the hygienic quality of the dishes cooked and sold by the women traders in the town of Korhogo. The study was carried out with 45 restaurants in the town of Korhogo. It consisted on the one hand, a survey based on the Ishikawa diagram in order to assess the hygienic conditions, and on the other hand of microbiological analyses of the ready-to-serve meals. A total of 90 samples were taken, including 2 dishes per restaurant, respectively consisting of one hot and one cold dish. Generally, results showed that hygienic conditions were unsatisfactory based on the utilized guidelines. From a microbiological point of view, the meal samples were 75.2% satisfactory, 7.6% acceptable and 17.2% unsatisfactory. In view of these results, it appears essential to improve hygiene conditions by greater involvement of the official services involved in training these traders and monitoring compliance with good hygiene practices and the establishment of a program of cleaning and disinfection of premises and equipment.</p> Karamoko Detto Yoro Dezay Thierry Moroh Jean-Luc Aboya Koffi Alex Joel Djè Koffi Marcellin ##submission.copyrightStatement## 2020-07-15 2020-07-15 1 10 10.9734/jamb/2020/v20i730258 Inhibitory Effect of Methanolic and Methanolic-Aqueous Mixture Extract of Leaves of Plectranthus neochilus Schltr (Lamiaceae) and Bauhinia rufescens Lam (Fabaceae) on Two Strains of Enterobacteria Producing Beta-lactamases <p>The objective of the study was to evaluate <em>in vitro</em> inhibitory effect of methanolic and methanolic-aqueous mixture extracts of <em>Plectranthus neochilus</em> Schltr (<em>P. neochilus</em>) and <em>Bauhinia rufescens</em> Lam (<em>B. rufescens</em>) on the growth of <em>Escherichia coli</em> 25922 and <em>Proteus mirabilis.</em> A phytochemical screening was carried out to highlight compounds (phenolic compounds, flavonoids, alkaloids) with antibacterial activity. Then, an antibiogram was Carried out to investigate the enzymes rendering the resistance. Finally, the E-test was used to evaluate the antibacterial activity of the extract mixture. The Screening results showed that both plants contain total phenolics, flavonoids and alkaloids compounds. The antibiogram has made it possible to establish the sensitivity profile of the strains tested with regard to certain antibiotics. The extract mixture showed antibacterial activity on both strains tested. In the present work, the different mixtures of extracts showed an inhibitory effect on <em>Escherichia coli </em>25922 [a strain sensitive to almost all the antibiotics tested, in particular the three classes: beta-lactams (Ceftazidine, Ceftriaxone, Meropenem), quinolones (Levofloxacin, Ciprofloxacin) and aminoglycosides (Gentamicin, Amikacin)] and on <em>Proteus mirabilis</em> (a multiresistant strain with almost all the antibiotics tested).</p> Mamoudou Hamadou Bakari Daoudou Baane Martin- Paul Salamatou Mohamadou Djoulde Darman Roger ##submission.copyrightStatement## 2020-07-18 2020-07-18 11 20 10.9734/jamb/2020/v20i730259 Rabies in Eswatini: What are the Issues and Challenges? <p>National efforts to control canine rabies have encountered some challenges, hence the disease is becoming endemic in Eswatini. The purpose of this study was to establish the extent to which resources and other relevant constraints may have an impact on implementing the national rabies programms in the country. In this study retrospective data going as far back as 1999 to 2018 was used to highlight the rabies situation in the country. The available insight into the existing and previous control measures may become useful guidelines for designing and implementing future control strategies.</p> <p>The major challenges identified were issues of vaccine and vaccination, government fiscal challenges, High birth rate of stray dogs were responsible for the rabies virus circulation in the domestic environment, provision of diagnostic facilities for testing of vaccine efficacy, vaccine quality, healthy dog carriers, check for vaccination antibody titres.</p> <p>Observations from this study were that the continuing efforts of the competent authority to spearhead the rabies control programms were acceptable and commendable considering the challenges encountered. A few recommendations are outlined for possible consideration as a contribution towards the rabies disease elimination.</p> B. N. Dlamini S. Mdluli C. Mudyanavana N. E. Chikuni M. T. Masarirambi ##submission.copyrightStatement## 2020-07-21 2020-07-21 21 28 10.9734/jamb/2020/v20i730260 Effect of Storage on the Nutritional Composition and Mycoflora of Sphenostylis stenocarpa Stored for Twenty Weeks <p>The nutritional and mycoflora changes in dried African yam beans (<em>Sphenostylis stenocarpa</em>) were investigated during a storage period of twenty weeks. The result of the proximate analysis (g/100 g) showed that the following moisture content decreased from 11.15-9.80, fat 1.25-0.49, crude fibre 6.18-2.64, crude protein 77.92-62.14 while the ash content increased from 3.20-3.78, carbohydrate content 0.36-18.32. The mineral analysis (mg/100 g) showed a decrease in all parameters investigated, sodium (Na) 2.57-1.35, potassium (K) 42.26-31.75, calcium (Ca) 21.45-12.56, magnesium (Mg) 27.75-20.72, iron (Fe) 0.25-0.12, zinc (Zn) 0.06-0.05, copper (Cu) 0.03-0.03, phosphorus (P) 32.08-22.03, manganese (Mn) 0.11-0.06, chromium (Cr) 0.002-0.001. Lead (Pb), cadmium (Cd) and nickel (Ni) were not detected. Five fungi comprising four genera were isolated using direct plating, washing and dilution methods on Potato Dextrose Agar (PDA), and identified using their cultural and morphological features with reference to standard procedures. The fungi isolated were <em>Aspergillus</em> <em>niger</em>, <em>Aspergillus flavus, Penicillium </em>sp., <em>Mucor</em> sp. and <em>Phytophthora palmivora</em>. It can be concluded that storage encourages proliferation of mycoflora thereby leading to reduction in the nutritional and mineral composition of the stored sample.</p> Emmanuel Dayo Fagbohun Fakiyesi Ifeoluwa Samuel Ayodeji Segun Ogundahunsi ##submission.copyrightStatement## 2020-07-25 2020-07-25 29 37 10.9734/jamb/2020/v20i730263